We describe stromatolites forming at an altitude of 3570 m at the shore of a volcanic lake Socompa, Argentinean Andes. The water at the site of stromatolites formation is alkaline, hypersaline, rich in inorganic nutrients, very rich in arsenic, and warm (20–24°C) due to a hydrothermal input. The stromatolites do not lithify, but form broad, rounded and low-domed bioherms dominated by diatom frustules and aragonite micro-crystals agglutinated by extracellular substances. In comparison to other modern stromatolites, they harbour an atypical microbial community characterized by highly abundant representatives of Deinococcus-Thermus, Rhodobacteraceae, Desulfobacterales and Spirochaetes. Additionally, a high proportion of the sequences that could not be classified at phylum level showed less than 80% identity to the best hit in the NCBI database, suggesting the presence of novel distant lineages. The primary production in the stromatolites is generally high and likely dominated by Microcoleus sp. Through negative phototaxis, the location of these cyanobacteria in the stromatolites is controlled by UV light, which greatly influences their photosynthetic activity. Diatoms, dominated by Amphora sp., are abundant in the anoxic, sulfidic and essentially dark parts of the stromatolites. Although their origin in the stromatolites is unclear, they are possibly an important source of anaerobically degraded organic matter that induces in situ aragonite precipitation. To the best of our knowledge, this is so far the highest altitude with documented actively forming stromatolites. Their generally rich, diverse and to a large extent novel microbial community likely harbours valuable genetic and proteomic reserves, and thus deserves active protection. Furthermore, since the stromatolites flourish in an environment characterized by a multitude of extremes, including high exposure to UV radiation, they can be an excellent model system for studying microbial adaptations under conditions that, at least in part, resemble those during the early phase of life evolution on Earth.

In this paper, we report the presence of sedimentary microbial ecosystems in wetlands of the Salar de Atacama. These laminated systems, which bind, trap and precipitate mineral include: microbial mats at Laguna Tebenquiche and Laguna La Brava, gypsum domes at Tebenquiche and carbonate microbialites at La Brava. Microbial diversity and key biogeochemical characteristics of both lakes (La Brava and Tebenquiche) and their various microbial ecosystems (non-lithifying mats, flat and domal microbialites) were determined. The composition and abundance of minerals ranged from trapped and bound halite in organic-rich non-lithifying mats to aragonite-dominated lithified flat microbialites and gypsum in lithified domal structures. Pyrosequencing of the V4 region of the 16s rDNA gene showed that Proteobacteria comprised a major phylum in all of the microbial ecosystems studied, with a marked lower abundance in the non-lithifying mats. A higher proportion of Bacteroidetes was present in Tebenquiche sediments compared to La Brava samples. The concentration of pigments, particularly that of Chlorophyll a, was higher in the Tebenquiche than in La Brava. Pigments typically associated with anoxygenic phototrophic bacteria were present in lower amounts. Organic-rich, non-lithifying microbial mats frequently formed snake-like, bulbous structures due to gas accumulation underneath the mat. We hypothesize that the lithified microbialites might have developed from these snake-like microbial mats following mineral precipitation in the surface layer, producing domes with endoevaporitic communities in Tebenquiche and carbonate platforms in La Brava. Whereas the potential role of microbes in carbonate platforms is well established, the contribution of endoevaporitic microbes to formation of gypsum domes needs further investigation.

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ABSTRACT. Despite the abundant fossil content of the Mata Amarilla Formation (Southern Patagonia, Santa Cruz Province, Argentina), its age has always generated a considerable number of questions and debates. The chronological data provided by invertebrates, dinosaurs, fish, turtles, plesiosaurs and fossil flora are contradictory. In this work, twenty U-Pb spot analyses by laser ablation were carried out on the outer parts of the zoned zircon crystals from a tuff layer of the middle section of the Mata Amarilla Formation, yielding a U-Pb concordia age of 96.23±0.71 Ma, which corresponds to the middle Cenomanian. The deposition of the lower section of the Mata Amarilla Formation marks the onset of the foreland stage of the Austral Basin (also known as Magallanes Basin); this transition is characterized by the west-east shift of the depositional systems, which is consistent with the progradation of the Cretaceous fold-and-thrust belt. Thus, the onset of the foreland stage could have occurred between the upper Albian and lower Cenomanian, as the underlying Piedra Clavada Formation is lower Albian in age. On comparing the data obtained with information from the Última Esperanza Province in Chile, it can be suggested that the initiation of the closure of the Rocas Verdes Marginal Basin occurred simultaneously. RESUMEN. Edades U-Pb en circones de la FormaciónMata Amarilla (Cretácico), Patagonia Austral, Argentina: su relación con la evolución de la Cuenca Austral. A pesar del abundante contenido fosilífero de la Formación Mata Amarilla (Patagonia Austral, Provincia de Santa Cruz, Argentina), siempre se generaron abundantes dudas y debates acerca de cuál es la edad de esta formación. Los datos cronológicos aportados por los invertebrados, los dinosaurios, peces, tortugas, plesiosaurios y flora fósil son dispares. En el presente trabajo se obtuvo una edad U-Pb concordia por la metodología de ablación láser aplicada a 20 puntos de la parte externa de circones zonados provenientes de un nivel tobáceo hallado en la sección media de la Formación Mata Amarilla, lo cual arrojó una edad de 96,23±0,71 Ma, que corresponde al Cenomaniano medio. La depositación de la sección inferior de la Formación Mata Amarilla marca el inicio del estadio de antepaís de la Cuenca Austral (también conocida como Cuenca de Magallanes); este pasaje está signado por el cambio oeste-este de los sistemas depositacionales, los cuales se encuentran en concordancia con la progradación de la faja plegada y corrida cretácica. Así, el comienzo del estadio de antepaís quedaría comprendido entre el Albiano superior y el Cenomaniano inferior, debido a que la subyacente Formación Piedra Clavada posee una edad Albiano inferior. Al comparar estos resultados con los datos de la Provincia de Última Esperanza en Chile, se sugiere que el comienzo del cierre de la cuenca marginal de Rocas Verdes se produjo en forma simultánea.

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The Central Andes in northern Chile contains a large number of closed basins whose central depression is occupied by saline lakes and salt crusts (salars). One of these basins is Salar de Llamara (850 m a.s.l.), where large domed structures of seemingly evaporitic origin forming domes can be found. In this work, we performed a detailed microbial characterization of these domes. Mineralogical studies revealed gypsum (CaSO(4)) as a major component. Microbial communities associated to these structures were analysed by 454 16S rDNA amplicon sequencing and compared between winter and summer seasons. Bacteroidetes Proteobacteria and Planctomycetes remained as the main phylogenetic groups, an increased diversity was found in winter. Comparison of the upper air-exposed part and the lower water-submerged part of the domes in both seasons showed little variation in the upper zone, showing a predominance of Chromatiales (Gammaproteobacteria), Rhodospirillales (Alphaproteobacteria), and Sphingobacteriales (Bacteroidetes). However, the submerged part showed marked differences between seasons, being dominated by Proteobacteria (Alpha and Gamma) and Verrucomicrobia in summer, but with more diverse phyla found in winter. Even though not abundant by sequence, Cyanobacteria were visually identified by scanning electron microscopy (SEM), which also revealed the presence of diatoms. Photosynthetic pigments were detected by high-performance liquid chromatography, being more diverse on the upper photosynthetic layer. Finally, the system was compared with other endoevaporite, mats microbialite and Stromatolites microbial ecosystems, showing higher similitude with evaporitic ecosystems from Atacama and Guerrero Negro. This environment is of special interest for extremophile studies because microbial life develops associated to minerals in the driest desert all over the world. Nevertheless, it is endangered by mining activity associated to copper and lithium extraction; thus, its environmental protection preservation is strongly encouraged.