Characterization of silver nanoparticle-producing bacteria from Tembagapura, Papua, Indonesia soil samples isolates has been investigated. Bacteria characterization was carried out macroscopically, microscopically, biochemically and molecularly. Furthermore, the results of the formed silver nanoparticles were characterized using UV-VIS spectroscopy, Fourier transform infrared (FTIR) and scanning electron microscopy (SEM). The experimental results showed maximum absorbance at 414 nm in TP10-1 isolates in UV-Vis spectroscopy. FTIR spectra of silver nanoparticles samples of TP10-1 isolates showed strong peaks in wave numbers 1637.65 cm-1 and 3329.47 cm-1. SEM micrographs reveal the formation of well dispersed silver nanoparticles. Silver nanoparticles of TP10-1 isolates that was measured by the imageJ program had an average particle size of 16,991 nm. Bacterial isolates with TP10-1 sample code which are identical to the Bacillus cereus strain GCF1I2 was able to synthesize silver nanoparticles.
The effectiveness of South Sumatra coffee extract cream in burn wound recovery of white male mice had been analyzed. This research aims to ensure which one between immature and mature coffee bean that was better to recover burn wounds of male white mice after 14-day testing. The prepared concentrations for both coffee beans were 0.1%, 0.2%, and 0.3%. We then figured out that ethanol contained by young and old coffee beans had a recovery effect on burn wounds of male white mice (Mus musculus). Findings indicate that 0.3% of mature coffee beans were more effective in recovering burn wounds of male white mice (Mus musculus), in which the wound recovery percentage was close to that in the positive control (burnazin).
VCO (virgin coconut oil) has benefits for skin health such as softening the skin, maintaining skin health, and protecting the skin from radiation and free radicals. VCO as one of the raw materials for making cream preparations can affect its physical stability. This study aimed to analyze the effect of VCO on the physical stability of sweet potato leaf extract creams and antibacterial activity test against Staphylococcus aureus ATCC 25923. The creams were made with five formulas namely base, extract cream 2%, and VCO 1%, 3%, 5% added to the extract cream 2% respectively. Physical stability test included storage for six weeks at room temperature. Stability parameters were organoleptic observation, homogeneity, pH, spreadability, adhesivity, and cream type. Statistical analysis was carried out with the Anova and Kruskal Wallis tests. The organoleptic test results of the cream extract showed a distinctive odor, green color, with semisolid and homogeneous texture. Increasing the concentration of VCO will increase the spreadability of extract cream but did not affect pH and adhesion. The conclusion of this study was that extract cream 2% without the addition of VCO was the most stable cream formulation. Besides that, all the cream formulas did not show antibacterial activity against Staphylococcus aureus ATCC 25923.
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