Background Amoxicillin-resistant Helicobacter pylori (H. pylori) strains seem to have increased over time in Vietnam. This threatens the effectiveness of H. pylori eradication therapies with this antibiotic. This study aimed to investigate the prevalence of primary resistance of H. pylori to amoxicillin and to assess its association with pbp1A point mutations in Vietnamese patients. Materials and methods Naive patients who presented with dyspepsia undergoing upper gastrointestinal endoscopy were recruited. Rapid urease tests and PCR assays were used to diagnose H. pylori infection. Amoxicillin susceptibility was examined by E-tests. Molecular detection of the mutant pbp1A gene conferring amoxicillin resistance was carried out by real-time PCR followed by direct sequencing of the PCR products. Phylogenetic analyses were performed using the Tamura-Nei genetic distance model and the neighbor-joining tree building method. Results There were 308 patients (46.1% men and 53.9% women, p = 0.190) with H. pylori infection. The mean age of the patients was 40.5 ± 11.4 years, ranging from 18 to 74 years old. The E-test was used to determine the susceptibility to amoxicillin (minimum inhibitory concentration (MIC) ≤ 0.125 μg/ml) in 101 isolates, among which the rate of primarily resistant strains to amoxicillin was 25.7%. Then, 270 sequences of pbp1A gene fragments were analysed. There were 77 amino acid substitution positions investigated, spanning amino acids 310–596, with the proportion varying from 0.4 to 100%. Seven amino acid changes were significantly different between amoxicillin-sensitive (AmoxS) and amoxicillin-resistant (AmoxR) samples, including Phe366 to Leu (p <  0.001), Ser414 to Arg (p <  0.001), Glu/Asn464–465 (p = 0.009), Val469 to Met (p = 0.021), Phe473 to Val (p <  0.001), Asp479 to Glu (p = 0.044), and Ser/Ala/Gly595–596 (p = 0.001). Phylogenetic analyses suggested that other molecular mechanisms might contribute to amoxicillin resistance in H. pylori in addition to the alterations in PBP1A. Conclusions We reported the emergence of amoxicillin-resistant Helicobacter pylori strains in Vietnam and new mutations statistically associated with this antimicrobial resistance. Additional studies are necessary to identify the mechanisms contributing to this resistance in Vietnam.

Tolerance and resistance are complex biological phenotypes that are desirable bioengineering goals for those seeking to design industrial strains or prevent the spread of antibiotic resistance. Over decades of research, a wealth of information has been generated to attempt to decode a molecular basis for tolerance, but to fully achieve the goal of engineering tolerance, researchers must be able to easily learn from a variety of data sources. To this end, we here describe a resource designed to enable scrutiny of diverse tolerance phenotypes. We have curated hundreds of gene expression studies exploring the response of Escherichia coli to chemical and environmental perturbations, from antibiotics to biofuels and solvents and more. Overall, our efforts give rise to a database encompassing more than 56 000 gene expression changes across 89 different stress conditions. This resource is designed for compatibility with the Resistome database, which includes more than 5000 strains with mutations conferring resistance or sensitivity but no transcriptomic data. Thus, the work here results in the first combined resource specialized to tolerance and resistance in E. coli that supports investigations across genomic, transcriptomic, and phenotypic levels. We leverage the database to identify promising bioengineering targets by searching globally across multiple stress conditions as well as by narrowing the focus to fewer conditions of interest, such as biofuel stress and antibiotic stress. We discuss some of the most frequently differentially expressed or coexpressed genes, and predict which transcription factors and sigma factors most likely contribute to gene expression profiles in a wide array of conditions. We also compare profiles from sensitive and resistant strains, gaining knowledge of how responses differ per overrepresented gene ontology terms. Finally, we search for genes that are frequently differentially expressed but not mutated, with the expectation that these may present interesting targets for future engineering efforts. The curated data presented here is publicly available, and should be advantageous to those studying a variety of bacterial tolerance phenotypes.

This study presents a procedure for calculating the change of the safety factor for unsaturated slopes of homogenous, residual soils suffering from rainfall infiltration within Khanh Vinh district, Khanh Hòa province. Rainfall is supposed as a main trigger caused failure of the potential sliding slopes. Rainwater into the slope due to infiltration caused an increase in moisture content and negative pore water pressure; a decrease in matric suction and in shear strength on the failure surface. Thus, slopes are reduced stability and can be failed. Soil permeability and rainfall intensity were found to be the primary factors controlling the instability of slopes due to rainfall, while the initial water table location and slope geometry only played a secondary role. A numerical model of analysis coupled seepage-stability used to simulate the seepage and slope stability under conditions of specific environment such as soil permeability, rainfall intensity, water table location and slope geometry in the study area. The relationships between safety factor and rainfall intensity, soil permeability, angle slope, high slope were identified to provide a good indication for the management of landslide hazards under the effects of rainfall.

The purpose of this study is to produce landslide hazard map in Khanh Vinh district, Khanh Hoa province using logistic regression method integrated with GIS analytical tools. The spatial relationship between landslide-related factors such as topography; lithology; vegetation; maximum precipitation in year; distance from roads; distance from drainages; distance from faults and the distribution of landslides were used in the landslide hazard analyses. Using success rate and prediction rate curve assess the fit and accuracy of logistic regression method. The results show that this method have the goodness of fit and the high accuracy (Areas Under Curves - AUC = 0.8 ~ 0.9). Bayesian Model Average (BMA) of the R statistical software was applied to identify the most influential factors and the combinatorial optimization models of landslide-related factors. There are four the most important landslide-related factors and five combinatorial optimization models of landslide-related factors. Model 3 (slope angle, slope aspect, altitude, distance from roads and maximum precipitation in year) is the best optimization.

In this paper, a Monte Carlo simulation used to analyze probabilistic slope stability. The results including: probabilistic slope failure and reliability index with respect to factor of safety under the effects of uncertainties in the parameters of soil properties. Base on this informations, geotechnical engineers how to get optimal designs to prevent slope failure. In addition, the purpose of this paper is to show that standard deviation of soil properties can be applied in simple ways, without more data, time, or effort than are commonly available in geotechnical engineering practice. Applying Monte Carlo simulation to evaluate probabilistic slope stability on route Nha Trang - Da Lat.

In this work, pyrolytic carbon electrodes were prepared through pyrolysis of well-patterned AZ 1505 positive photoresist films. The designed electrodes firstly were prepared via photolithography technique, then the polymer was thermally broken-down into carbon skeletons in an oxygen-free environment using pyrolysis technique. The effect of the highest temperature and ramping rate on the electrical properties of the carbon films were investigated. The results show that the pyrolysis process was optimal at the ramping rate of 3 °C/minute, annealing temperature of 900 °C, and annealing time of one hour. The lowest resistivity was obtained at 6.3 ´ 10-5 Wm for pyrolytic films prepared at the optimal pyrolysis conditions. Electrochemical measurements confirm the potential of this electrode for electrochemical sensing applications.

Background Amoxicillin resistant Helicobacter pylori (H. pylori) strains seem to have increased over time in Vietnam. This threatens the effectiveness of H. pylori eradication therapies with this antibiotic. This study aimed to investigate the prevalence of primary resistance of H. pylori to amoxicillin and to assess its association with pbp1A point mutations in Vietnamese patients. Materials and Methods Naive patients who presented with dyspepsia undergoing upper gastrointestinal endoscopy were recruited. Rapid urease tests and PCR assays were used to diagnose H. pylori infection. Amoxicillin susceptibility was examined by E-tests. Molecular detection of the mutant pbp1A gene conferring amoxicillin resistance was carried out by real-time PCR followed by direct sequencing of the PCR products. Phylogenetic analyses were performed using the Tamura-Nei genetic distance model and the neighbour-joining tree building method. Results There were 308 patients (46.1% men and 53.9% women, p = 0.190) with H. pylori infection. The mean age of the patients was 40.5 ± 11.4 years, ranging from 18 to 74 years old. The E-test was used to determine the susceptibility to amoxicillin (minimum inhibitory concentration (MIC) ≤ 0.125 µg/ml) in 101 isolates, among which the rate of primarily resistant strains to amoxicillin was 25.7%. Then, 270 sequences of pbp1A gene fragments were analysed. There were 77 amino acid substitution positions investigated, spanning amino acids 310–596, with the proportion varying from 0.4–100%. Seven amino acid changes were significantly different between amoxicillin-sensitive (AmoxS) and amoxicillin-resistant (AmoxR) samples, including Phe366 to Leu (p < 0.001), Ser414 to Arg (p < 0.001), Glu/Asn464−465 (p = 0.009), Val469 to Met (p = 0.021), Phe473 to Val (p < 0.001), Asp479 to Glu (p = 0.044), and Ser/Ala/Gly595−596 (p = 0.001). Phylogenetic analyses suggested that other molecular mechanisms might contribute to amoxicillin resistance in H. pylori in addition to the alterations in PBP1A. Conclusions We reported the emergence of amoxicillin-resistant Helicobacter pylori strains in Vietnam and new mutations statistically associated with this antimicrobial resistance. Additional studies are necessary to identify the mechanisms contributing to this resistance in Vietnam.

No abstract