Oleaginous yeast Yarrowia lipolytica is an important host for the production of lipidderived compounds or heterologous proteins. Selection of strong promoters and effective expression systems is critical for heterologous protein secretion. To search for a strong promoter in Y. lipolytica, activities of FBA1, TDH1 and GPM1 promoters were compared to that of TEF1 promoter by constructing GUS reporter fusions. The FBA1 promoter activity was 2.2 and 5.5 times stronger than the TDH1 and GPM1 promoters, respectively. The FBA1 IN promoter (FBA1 sequence of À826 to +169) containing an intron (+64 to +165) showed five-fold higher expression than the FBA1 promoter (À831 to À1). The transcriptional enhancement by the 5′-region within the FBA1 gene was confirmed by GPM1::FBA1 chimeric promoter construction. Using the strong FBA1 IN promoter, four different S. cerevisiae SUC2 expression cassettes were tested for the SUC + phenotype in Y. lipolytica. Functional invertase secretion was facilitated by the Xpr2 prepro-region with an additional 13 amino acids of mature Xpr2, or by the native Suc2 signal sequence. However, these two secretory signals in tandem, or the mature Suc2 with no secretory signal, did not direct secretion of functional invertase. Unlike previously reported Y. lipolytica SUC + strains, our engineered stains secreted most of invertase into the medium. Copyright