These data provide the first in vivo demonstration of a site-specific ultrasonic contrast agent and have potential for improved sensitivity and specificity for noninvasive diagnosis of thrombi and other pathological diseases.
Background-Restenosis is a serious complication of coronary angioplasty that involves the proliferation and migration of vascular smooth muscle cells (VSMCs) from the media to the intima, synthesis of extracellular matrix, and remodeling. We have previously demonstrated that tissue factor-targeted nanoparticles can penetrate and bind stretch-activated vascular smooth muscles in the media after balloon injury. In the present study, the concept of VSMC-targeted nanoparticles as a drug-delivery platform for the prevention of restenosis after angioplasty is studied. Methods and Results-Tissue factor-targeted nanoparticles containing doxorubicin or paclitaxel at 0, 0.2, or 2.0 mole% of the outer lipid layer were targeted for 30 minutes to VSMCs and significantly inhibited their proliferation in culture over the next 3 days. Targeting of the nanoparticles to VSMC surface epitopes significantly increased nanoparticle antiproliferative effectiveness, particularly for paclitaxel. In vitro dissolution studies revealed that nanoparticle drug release persisted over one week. Targeted antiproliferative results were dependent on the hydrophobic nature of the drug and noncovalent interactions with other surfactant components. Molecular imaging of nanoparticles adherent to the VSMC was demonstrated with high-resolution T 1 -weighted MRI at 4.7T. MRI 19 F spectroscopy of the nanoparticle core provided a quantifiable approach for noninvasive dosimetry of targeted drug payloads. Conclusions-These data suggest that targeted paramagnetic nanoparticles may provide a novel, MRI-visualizable, and quantifiable drug delivery system for the prevention of restenosis after angioplasty.
A biodegradable positron-emitting dendritic nanoprobe targeted at ␣v3 integrin, a biological marker known to modulate angiogenesis, was developed for the noninvasive imaging of angiogenesis. The nanoprobe has a modular multivalent core-shell architecture consisting of a biodegradable heterobifunctional dendritic core chemoselectively functionalized with heterobifunctional polyethylene oxide (PEO) chains that form a protective shell, which imparts biological stealth and dictates the pharmacokinetics. Each of the 8 branches of the dendritic core was functionalized for labeling with radiohalogens. Placement of radioactive moieties at the core was designed to prevent in vivo dehalogenation, a potential problem for radiohalogens in imaging and therapy. Targeting peptides of cyclic arginine-glycine-aspartic acid (RGD) motifs were installed at the terminal ends of the PEO chains to enhance their accessibility to ␣v3 integrin receptors. This nanoscale design enabled a 50-fold enhancement of the binding affinity to ␣v3 integrin receptors with respect to the monovalent RGD peptide alone, from 10.40 nM to 0.18 nM IC50. Cell-based assays of the 125 I-labeled dendritic nanoprobes using ␣v3-positive cells showed a 6-fold increase in ␣v3 receptor-mediated endocytosis of the targeted nanoprobe compared with the nontargeted nanoprobe, whereas ␣v3-negative cells showed no enhancement of cell uptake over time. In vivo biodistribution studies of 76 Brlabeled dendritic nanoprobes showed excellent bioavailability for the targeted and nontargeted nanoprobes. In vivo studies in a murine hindlimb ischemia model for angiogenesis revealed high specific accumulation of 76 Br-labeled dendritic nanoprobes targeted at ␣v3 integrins in angiogenic muscles, allowing highly selective imaging of this critically important process.dendrimer ͉ molecular imaging
Recently, we have identified the dramatic depletion of cardiolipin (CL) in diabetic myocardium 6 weeks after streptozotocin (STZ) injection that was accompanied by increases in triacylglycerol content and multiple changes in polar lipid molecular species. However, after 6 weeks in the diabetic state, the predominant lipid hallmarks of diabetic cardiomyopathy were each present concomitantly, and thus, it was impossible to identify the temporal course of lipid alterations in diabetic myocardium. Using the newly developed enhanced shotgun lipidomics approach, we demonstrated the dramatic loss of abundant CL molecular species in STZ-treated hearts at the very earliest stages of diabetes accompanied by a profound remodeling of the remaining CL molecular species including a 16-fold increase in the content of 18:2-22:6-22:6-22:6 CL. These alterations in CL metabolism occur within days after the induction of the diabetic state and precede the triacylglycerol accumulation manifest in diabetic myocardium. Similarly, in ob/ob mice, a dramatic and progressive redistribution from 18:2 FA-containing CL molecular species to 22:6 FA-containing CL molecular species was also identified. Collectively, these results demonstrate alterations in CL hydrolysis and remodeling at the earliest stages of diabetes and are consistent with a role for alterations in CL content in precipitating mitochondrial dysfunction in diabetic cardiomyopathy.Diabetic cardiomyopathy is characterized by the presence of marked alterations in the lipid composition of myocardium, inefficient substrate utilization, and diastolic dysfunction (1-9). Many studies have implicated mitochondrial dysfunction (1,2,4,5,(7)(8)(9)(10)(11) as the underlying mechanism that precipitates hemodynamic dysfunction and contributes to the untoward sequelae of events in diabetic patients following myocardial ischemia. Persistent changes in substrate utilization occur in diabetic myocardium with an increased utilization of fatty acid substrate and a decreased dependence on glucose. Increased fatty acid utilization promotes the generation of reactive oxygen species which can oxidize highly unsaturated lipids in the mitochondrial compartment such as cardiolipin (CL) 1 and impair mitochondrial function. Collectively, these features each contribute to the accumulation of toxic lipids in diabetic myocardium [e.g., acylcarnitines, acyl-CoAs, and triacylglycerols (TG)] that compromise the functional integrity of many membrane systems (2,4,5,7,9). In early studies, we and others identified profound alterations in the myocardial lipid composition in obese and diabetic rats, which were accompanied by physiologic dysfunction (1, 2). The abnormalities in lipid metabolism present in diabetic myocardium include the accumulation of acylcarnitines, thereby directly implicating mitochondrial dysfunction as a likely contributing mechanism to the compromised metabolic and hemodynamic efficiency of diabetic myocardium. The recent appreciation of these processes has led to the widespread agreem...
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