Background The use of total knee arthroplasty (TKA) in treatment of chronic degenerative pathologies of the knee has boasted of an experience of 50 years. The aim of this bibliometric and visualized study is to comprehensively examine the current status and global trends of TKA research. Methods Publications related to TKA research from 2010 to 2019 were retrieved from Web of Science database, and then analyzed through bibliometric methodology. As for the visualized study, the software VOS viewer was utilized for bibliographic coupling, coauthorship, cociation, and co-occurrence analysis, along with further simulation of publication trends in this field. Results A total of 8631 publications were eventually included. The number of publications tends to increased annually over the worldwide. The United States was the pioneer which has made tremendous contribution, with the most publications and citations, as well as the highest H-index. The JOURNAL OF ARTHROPLASTY has published most papers, while CLINICAL ORTHOPAEDICS AND RELATED RESEARCH has the highest citation frequency. Hospital for Special Surgery have made the greatest contribution when total publication number and coauthorship were taken together. Studies could be divided into five clusters: “alignment study”, “revision TKA study”, “complication study”, “rehabilitation study”, and “perioperative management study”, which have a trend of balanced development in this field. Conclusions There will be an increasing number of publications on TKA research according to the current global trends, and the United States maintained the leadership in this area. Additionally, a trend of balanced development may exist in the field of TKA research, accompanied with inherent changes of hotspots in each cluster.
Purpose: MiRNA-381 is known to regulate osteoarthritic cartilage degeneration in our previous study, but its target remains unclear. The aim of this study is to identify histone deacetylase 4 (HDAC4), an inhibitor of chondrocyte hypertrophy, as a target of miRNA-381. Methods: The long bone of forearm in mouse embryo served as an in vivo model of chondrogenesis and hypertrophy. The expression of miRNA-381was determined by in situ hybridization, while HDAC4 and MMP13 levels were detected by immunohistochemistry.SW1353 chondrocyte-like cells and primary mouse chondrocytes (PMCs) was transfected with miRNA-381 mimic or miRNA-381 inhibitor in vitro. HDAC4 siRNA was used to determine its function in chondrocyte differentiation and hypertrophy. Direct interaction between miR-381 and putative site in the 3 0-untranslated region (3 0-UTR) of HDAC4 messenger RNA was validated by luciferase reporter assay. Results: MiRNA-381, RUNX2 and MMP13 were mainly expressed in hypertrophic chondrocytes, while HDAC4 showed a high expression in pre-hypertrophicchondrocytes and decreased apparently in hypertrophic zone. Overexpression of miRNA-381 suppressed the activity of a reporter construct containing the 3 0-UTR, decreased HDAC4 expression and increased acetylated-Histone H3, RUNX2 and MMP13. Down-regulaiton of HDAC4 using siRNA significantly increased RUNX2 expression. Conclusions: MiRNA381 epigenetically regulates chondrocyte hypertrophy and degeneration by directly targeting HDAC4.
Background: To assess the association of co-existing MRI lesions with knee pain at rest or on joint loading.Methods: We included participants from Osteoarthritis Initiative whose pain score, measured by WOMAC sub-scales, differed by ≥1 point at rest (in bed at night, sitting/lying down) or on joint loading (walking, stairs) between two knees. Cartilage morphology, bone marrow lesions, meniscus extrusion, meniscus morphology, Hoffa’s synovitis and synovitis-effusion were assessed using the compartment-specific MRI Osteoarthritis Knee Score. We performed latent class analyses to identify subgroups of co-existing MRI lesions and fitted a conditional logistic regression model to examine their associations with knee pain. Results: Among 130 eligible participants, we identified five subgroups of knees according to patterns of co-existing MRI lesions: I. minimal lesions; II. mild lesions; III. moderate morphological lesions; IV. moderate multiple reactive lesions; and V. severe lesions. Compared with subgroup I, the odds ratios (ORs) and 95% confidence intervals (CI) of greater pain in bed at night were 1.6 (0.3, 7.2), 2.2 (0.5, 9.5), 6.2 (1.3, 29.6) and 11.2 (2.1, 59.2) for subgroups II-V, respectively. A similar association was observed between aforementioned subgroups and pain with sitting/lying down. The ORs (95% CI) of greater pain with walking were 1.0 (reference), 1.7 (0.5, 6.1), 0.7 (0.2, 2.3), 5.0 (1.4, 18.6) and 7.9 (2.0, 31.5) for subgroup I-V, respectively. The corresponding analysis for pain on stairs showed similar results. Conclusions: Distinct patterns of co-existing MRI lesions have different implications for the pathogenesis of osteoarthritic knee pain occurring with/without joint loading.
Background: Long noncoding RNAs (lncRNAs) are recently found to be critical regulators of the epigenome. However, our knowledge of their role in osteoarthritis (OA) development is limited. This study investigates the mechanism by which HOTAIR, a key lncRNA with elevated expression in OA, affects OA disease progression. Results: HOTAIR expression was greatly elevated in osteoarthritic compared to normal chondrocytes. Silencing and over-expression of HOTAIR in SW1353 cells respectively reduced and increased the expression of genes associated with cartilage degradation in OA. Investigation of molecular pathways revealed that HOTAIR acted directly on Wnt inhibitory factor 1 (WIF-1) by increasing histone H3K27 trimethylation in the WIF-1 promoter, leading to WIF-1 repression that favours activation of the Wnt/β-catenin pathway. Conclusions: Activation of Wnt/β-catenin signalling by HOTAIR through WIF-1 repression in osteoarthritic chondrocytes increases catabolic gene expression and promotes cartilage degradation. This is the first study to demonstrate a direct link between HOTAIR, WIF-1 and OA progression, which may be useful for future investigations into disease biomarkers or therapeutic targets.
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