Background: To investigate the role and underlying mechanism of cyclin G2 (G2-type cyclin) in the formation of vascular smooth muscle cells (VSMCs) derived foam cells. Methods:The levels of α-SMA (alpha-SM-actin), p-NF-κB (phosphorylation nuclear transcription factors kappa B), and LOX-1 (lectin-like oxidized low-density lipoprotein receptor-1) were measured by immunohistochemistry and western blotting. The mouse aortic root smooth muscle cell line MOVAS was transfected to over-express cyclin G2, which were then stimulated with 80 μg/mL ox-LDL (oxidized lowdensity lipoprotein) to induce foam cell formation. DT-061 an activator of PP2A (protein phosphatase 2A) agonist was used to verify the role of PP2A in the process.Results: Knocking out the Ccng2 gene in Apoe -/mice alleviated aortic lipid plaque, foam cell formulation, ameliorative body weight, and LDL-cholesterol. We observed that the number of α-SMA positive cells was significantly decreased in Apoe -/-Ccng 2-/mice compared to Apoe -/mice. Also, the protein levels of p-NF-κB and LOX-1 were markedly reduced in the aortic root of Apoe -/-Ccng2 -/mice. Upon stimulation with ox-LDL, upregulated cyclin G2 increased the intracellular lipid accumulation in MOVAS cells. Also, it suppressed the activity of PP2A but up-regulated LOX-1. Additionally, the cell nuclear translocation of p-NF-κB was increased. Interestingly, DT-061 intervention, re-activating the activity of PP2A, reduced the levels of nuclear p-NF-κB and LOX-1. This led to decreased lipid endocytosis reducing the formation of VSMCsderived foam cells.Conclusions: Cyclin G2 increases the nuclear translocation of p-NF-κB by reducing the enzymatic activity of PP2A and upregulating LOX-1, thereby promotes the formation of VSMCs -derived foam cells in atherosclerosis.
For storage and recovery requirements on large-scale seismic waveform data of the National Earthquake Data Backup Center (NEDBC), a distributed cluster processing model based on Kafka message queues is designed to optimize the inbound efficiency of seismic waveform data stored in HBase at NEDBC. Firstly, compare the characteristics of big data storage architectures with that of traditional disk array storage architectures. Secondly, realize seismic waveform data analysis and periodic truncation, and write HBase in NoSQL record form through Spark Streaming cluster. Finally, compare and test the read/write performance of the data processing process of the proposed big data platform with that of traditional storage architectures. Results show that the seismic waveform data processing architecture based on Kafka designed and implemented in this paper has a higher read/write speed than the traditional architecture on the basis of the redundancy capability of NEDBC data backup, which verifies the validity and practicability of the proposed approach.
PurposeThis paper presents a preliminary study on whether repetitive transcranial magnetic stimulation (rTMS) can modulate the nutritional status of persistent vegetative state (PVS) patients (the primary endpoint) by regulating the intestinal flora and the metabolites, with the correlation between them also investigated.MethodsSeventy-six patients with PVS were selected and divided into the observation group (n = 38) and the control group (n = 38) by random numerical grouping. All subjects’ stool samples were examined for metabolites and analyzed regarding the short-chain fatty acids (SCFAs) content. All subjects’ serum albumin, prealbumin, and hemoglobin levels were measured before and after the treatment. Nutrition risk screening 2002 was performed on all the subjects before and after the treatment and on the 30th and 90th days of the follow-up.Results(1) Intestinal flora structure: the Chao index, Ace index, and Shannon index of the observation group and the control group were significantly higher (p < 0.05), while the Simpson index was significantly lower (p < 0.05) following the treatment. (2) Metabolites of the intestinal flora: the observation group had significantly higher levels of acetic acid, butyric acid, and valeric acid (p < 0.05), as well as lower levels of propionic acid (p < 0.05) following the treatment. (3) Nutritional status (the primary endpoint): following the treatment, the above serum nutritional indices were significantly higher in both groups (p < 0.05), while the indices of the observation group were significantly higher than those of the control group (p < 0.05).ConclusionThe rTMS method may improve the nutritional status of patients with PVS by regulating the structure of the intestinal flora and affecting the level of SCFAs through the microbiota–gut–brain axis. The possible mechanism involves how high-frequency rTMS can cause increased excitation in the frontal lobe of the right side of the brain, thus regulating the 5-hydroxytryptamine and norepinephrine levels.
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