4591 From 2008 to date we marked all Chronic Lymphocytic Leukemia (CLL) with monoclonal CD34. The authors consider presence of said marker on the CLL cell membrane is a prognostic added factor to positivity for CD38 and ZAP70. CLL frequency is lower in countries with mixed races, compared with Europe and North America. In Venezuela is 5.4% (Luigi De Salvo personal communication) where I have never diagnosed a CLL case in a pure Venezuelan Indian. Similar reports are documented from Japan and China, provided racial genetics. Since 2008 to date we studied 28 patients for CLL: male 18 and female 10 with CD34-labeled. All patients presented lymphadenopathy, splenomegaly, and hypogammaglobulinemia. Flow cytometry revealed: HLA-Dr (33–100%), CD19 (44–100%), CD20 (28–95%), CD23 (63–100%), CD19 + CD5 (44–100%), CD23 + CD5 (28–100%) and CD19 + CD23 (28–100%) ZAP-70 (31–97%) and CD38 (12–75%). Of 28 patients studied, 21 individual CD34+ presented a variability of 10–99%. All had diffuse bone marrow infiltration, anemia and a white cell count range of 20.000–325,000/dl, with more than 80% lymphocytes of mature appearance. All patients presented discrete thrombocytopenia 70,000–120,000 plt/ml and responded well to standard treatment with Fludara + Rituximab. We believe presence of CD34 antigen on the cell membrane is an added risk factor for patients with CLL, producing an increased angiogenesis, and infiltration of organs and bone marrow by the leukemia cell. Lack of complete remission or negativity of Minimal Residual Disease may be CD34-linked. Disclosures: No relevant conflicts of interest to declare.
5101 We report a 23 yr female patient which relates beginning of current illness three months prior diagnosis; characterized with increased abdominal volume, concomitant active hemorrhagic manifestations consisting of ecchymosis and petechiae. Upon clinical, bone marrow biopsy, immunohistochemistry and subsequent surgery, case corresponded to Diffuse Large B-Cells Lymphoma(DLBCL) of splenic intravascular origin. Organ weight and size after splenectomy 2.53 Kg size 30×22×10 cm). Microscopic diagnosis description corresponds with presence of monomorphic lymphoid cells with predominance of small neoplasic lymphoid cells with small round nucleus, granular chromatin and small nucleolus accompanied with eventual cytoplasmic membrane irregularities. Presence of reactive plasmocytes and sever splenic congestion. The unusual case reported corresponds to an invasive lymphoma of splenic vascular origin. By our knowledge this is the first case register in Venezuela. Disclosures: No relevant conflicts of interest to declare.
4717 Research on stimulatory and inhibitory factors present in the hematopoietic microenvironment promoted development of ex-vivo cell culture methods. Recent investigations strive to select natural and novel mitogenic substances within defined conditions to culture and harvest specific progenitor cells and cell lines. Optimal large scale cell expansion of HSCs remains to be developed. The present study documents ex-vivo human lymphocytes and platelet culture in autologous human blood plasma (BP) complemented with a novel ionic metal polysulphide. Resulting plasma is growth-factor rich (FRP) with a high platelet count (HPC) containing > 4.5×10E6 plts/ul. Co-culture expansion for 3-days of normal peripheral blood cells from n=20 healthy individuals in FRP promotes lymphoid and platelet series stem cell lineage growth and differentiation. Cultured cells characterized using conventional hematology and flow cytometry methods on CD3, CD4, CD8, CD34, CD38, CD41, CD61 antigen bearing cells; and average values reported. Result analysis of CD3 and CD4 bearing cells before-after a 3-day culture indicates a 4 % and 18 % respective increase, while CD8 cell population decrease 7%. A stimulation response obtained on CD34 and CD38 cells with a 67.30% and 53.89 % respective increase. Cell growth of CD41 and CD61 antigen bearing cells increased 50.89 % and 69.22 %, respectively. Functional platelet counts (before-after culture) correspond with a 3–5 fold increase of original concentration, and presence of recently reported proto-platelets. Minimum and maximum values varied among studied population. Discrete elevation in Neutrophils and Granulocytes counts observed. Potential applications thought for immunosuppression, cancer and organ transplant. Disclosures: No relevant conflicts of interest to declare.
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