In the present work, five hundred and sixty-seven isolates of lactic acid bacteria were recovered from raw bee pollen grains. All isolates were screened for their antagonistic activity against both Gram-positive and Gram-negative pathogenic bacteria. Neutralized supernatants of 54 lactic acid bacteria (LAB) cultures from 216 active isolates inhibited the growth of indicator bacteria. They were phenotypically characterized, based on the fermentation of 39 carbohydrates. Using the simple matching coefficient and unweighted pair group algorithm with arithmetic averages (UPGMA), seven clusters with other two members were defined at the 79% similarity level. The following species were characterized: Lactobacillus plantarum, Lactobacillus fermentum, Lactococcus lactis, Pediococcus acidilactici, Pediococcus pentosaceus, and unidentified lactobacilli. Phenotypic characteristics of major and minor clusters were also identified. Partial sequencing of the 16S rRNA gene of representative isolates from each cluster was performed, and ten strains were assigned to seven species: Lactobacillus plantarum, Lactobacillus fermentum, Lactococcus lactis, Lactobacillus ingluviei, Pediococcus pentosaceus, Lactobacillus acidipiscis and Weissella cibaria. The molecular method used failed to determine the exact taxonomic status of BH0900 and AH3133.
The objective of this study was to evaluate different biological activities of aqueous and ethanolic extracts from Thymelaea microphylla. Antioxidant capacity was determined using two methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and the inhibition of lipid peroxidation using the β-carotene/linoleate model system. Disc diffusion method was used to evaluate the antibacterial activity on four different strains. Hypoglycemic effect of aqueous extract was tested in rats. Aqueous and ethanolic extracts showed a good antioxidant activity with IC50 of 0.1 and 0.2 mg/ml in DPPH test, and RRA% of 46.40% and 77.86% in β-carotene/linoleate assay respectively. Both extracts had no antibacterial effect on studied strains. A single administration of aqueous extract at a dose of 250 mg/kg bodyweight caused a very significant decrease in blood glucose level in rat. Keywords: Antibacterial activity, β-carotene/linoleate, DPPH, Hypoglycemic activity, Thymelaea microphylla Please cite this paper as: Dahamna S, Dehimi K, Merghem M, et al. Antioxidant, antibacterial and hypoglycemic activity of extracts from Thymelaea microphylla Coss. et Dur.
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