No abstract

Nanofibrillated cellulose, obtained from rice straw agricultural wastes was used as a substrate for the preparation of a new injectable and mineralized hydrogel for bone regeneration. Tetramethyl pyridine oxyl (TEMPO) oxidized nanofibrillated cellulose, was mineralized through the incorporation of a prepared and characterized biphasic calcium phosphate at a fixed ratio of 50 wt%. The TEMPO-oxidized rice straw nanofibrillated cellulose was characterized using transmission electron microscopy, Fourier transform infrared, and carboxylic content determination. The injectability and viscosity of the prepared hydrogel were evaluated using universal testing machine and rheometer testing, respectively. Cytotoxicity and alkaline phosphatase level tests on osteoblast like-cells for in vitro assessment of the biocompatibility were investigated. Results revealed that the isolated rice straw nanofibrillated cellulose is a nanocomposite of the cellulose nanofibers and silica nanoparticles. Rheological properties of the tested materials are suitable for use as injectable material and of nontoxic effect on osteoblast-like cells, as revealed by the positive alkaline phosphate assay. However, nanofibrillated cellulose/ biphasic calcium phosphate hydrogel showed higher cytotoxicity and lower bioactivity test results when compared to that of nanofibrillated cellulose.

No abstract

No abstract

Pumpkin seed oil was combined with polyvinyl alcohol (PVA) and chitosan (CS) to develop novel electrospun nanofibers that can serve as wound scaffolds. The main objective of this work is to evaluate the safety, drug release, and performance of these scaffolds for dermal and oral wound healing. The scaffolds were characterized using a field scanning electron microscope (FSEM), and Fourier transform infrared spectroscopy (FTIR). A series of tests were conducted, including the degree of swelling and degradation, in vitro drug release, antibacterial activity, and cytotoxicity in human dermal fibroblasts adult (HDFa). Wound healing activity in an excisional dermal and incisional oral wound's rat model was evaluated after 10 days. Antibacterial tests of the Pumpkin loaded CS/PVA nanofibers showed significant antibacterial activity against S. aureus and E. coli compared to negative controls. In vivo dermal wound healing showed that all treatment groups enhanced wound healing, as demonstrated by the increased wound closure percentages compared to negative control groups on 3, 5, and 10 days. Compared to other treatment groups, pumpkin-loaded CS/PVA nanofibers significantly enhanced healing on 7 and 10 days, however, healing was delayed afterward. In oral wound healing, the qualitative Hematoxylin and Eosin (HÀ E) histological examination of the oral wounds treated with pumpkin-loaded scaffolds showed an enhanced healing rate with healthy architecture compared to the wounds treated with unloaded scaffolds or untreated wounds. These results indicate that Pumpkin loaded nanofibers are promising for both dermal and oral wound healing. They offer a new safe and cheap alternative to the commercial treatments that are used for wound healing. The results also highlight the combination of using natural botanical compounds and emerging nanoscience for biomedical applications, primarily wound healing.