Four soybean storage protein subunit QTLs were mapped using bulked segregant analysis and an F population, which were validated with an F RIL population. The storage protein globulins β-conglycinin (7S subunit) and glycinin (11S subunits) can affect the quantity and quality of proteins found in soybean seeds and account for more than 70% of the total soybean protein. Manipulating the storage protein subunits to enhance soymeal nutrition and for desirable tofu manufacturing characteristics are two end-use quality goals in soybean breeding programs. To aid in developing soybean cultivars with desired seed composition, an F mapping population (n = 448) and an F RIL population (n = 180) were developed by crossing high protein cultivar 'Harovinton' with the breeding line SQ97-0263_3-1a, which lacks the 7S α', 11S A, 11S A, 11S A and 11S A subunits. The storage protein composition of each individual in the F and F populations were profiled using SDS-PAGE. Based on the presence/absence of the subunits, genomic DNA bulks were formed among the F plants to identify genomic regions controlling the 7S α' and 11S protein subunits. By utilizing polymorphic SNPs between the bulks characterized with Illumina SoySNP50K iSelect BeadChips at targeted genomic regions, KASP assays were designed and used to map QTLs causing the loss of the subunits. Soybean storage protein QTLs were identified on Chromosome 3 (11S A), Chromosome 10 (7S α' and 11S A), and Chromosome 13 (11S A), which were also validated in the F RIL population. The results of this research could allow for the deployment of marker-assisted selection for desired storage protein subunits by screening breeding populations using the SNPs linked with the subunits of interest.

On 11 July 1997 a very large debris flow occurred at Hummingbird Creek, Mara Lake, British Columbia. Long-term antecedent precipitation was record breaking, whereas short-term precipitation at Salmon Arm did not exceed the 2 year return period for intensity. A 25 000 m3 debris avalanche was initiated downstream of a forest road culvert, which drained a small catchment that had been artificially increased by a factor of three. The debris avalanche entered the channel of Hummingbird Creek and triggered a debris flow. Velocities were back-calculated using the forced vortex equation and multiplied by the cross-sectional area to obtain peak discharge estimates that ranged from 600 to 1000 m3/s. Approximately 92 000 m3 of sediment was deposited during this event, which makes it the largest nonvolcanic debris flow recorded in British Columbia to date. A three-dimensional runout model was used to simulate this event. Results are in fair agreement with the observed behaviour. This study emphasizes the need to carefully manage forest resources with high downstream risks and the need for hazard assessments prior to development on alluvial fans.Key words: debris avalanche, debris flow, DAN model, hydroclimatic, peak discharge, British Columbia.

Abstract.  1. This study evaluated the effect of dispersal on the density and feeding impact of a phytophagous insect in relation to the spatial distribution of its host plants.2. The interaction between density, dispersal, and feeding impact of western flower thrips on flowering chrysanthemum was quantified at three spatial scales, with infested and uninfested plants either isolated in 0.25 m2 individual cages, or enclosed together in 2.25 m2 communal cages or 75 m2 greenhouses.3. In individual cages, the rate of dispersal from chrysanthemum plants to blue sticky traps increased with the density of thrips for females but not males. Uninfested plants consistently had fewer thrips when they were individually caged rather than enclosed with plants infested with adults, indicating that dispersal mediates inter‐plant distribution of thrips.4. The feeding impact of thrips on inflorescences was evaluated using the absorbance of ethanol extracts at wavelengths characteristic of yellow carotenoid pigments associated with chrysanthemum inflorescences (415, 445, and 472 nm). Increasing absorbance of extracts with increasing density of thrips per inflorescence suggests that feeding by thrips results in ruptured cells leaching carotenoid pigments.5. In communal cages, the distribution of thrips was uniform for infested and uninfested plants, whereas the density and feeding impact of thrips in greenhouses were higher for infested than uninfested plants. These results suggest that short‐range dispersal by adults homogenises the density and feeding impact of thrips among host plants only on a small spatial scale.

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HS-182 and HS-183 are food-grade soybean lines [Glycine max (L.) Merr.] with distinct seed protein profiles and food processing quality. HS-182 is a 7S β-conglycinin α’ and 11S glycinin A4 null with a high protein concentration of 45.7% and good processing quality. HS-183 is a 7S β-conglycinin α’ and 11S glycinin null with a protein concentration of 42.7% and poor tofu processing quality. They are adapted to areas of southwestern Ontario with 3100 or more crop heat units and have relative maturity groups of 2.5 and 2.4, respectively.