Genetic mapping and validation of the loci controlling 7S α′ and 11S A-type storage protein subunits in soybean [Glycine max (L.) Merr.]

Abstract

Four soybean storage protein subunit QTLs were mapped using bulked segregant analysis and an F population, which were validated with an F RIL population. The storage protein globulins β-conglycinin (7S subunit) and glycinin (11S subunits) can affect the quantity and quality of proteins found in soybean seeds and account for more than 70% of the total soybean protein. Manipulating the storage protein subunits to enhance soymeal nutrition and for desirable tofu manufacturing characteristics are two end-use quality goals in soybean breeding programs. To aid in developing soybean cultivars with desired seed composition, an F mapping population (n = 448) and an F RIL population (n = 180) were developed by crossing high protein cultivar 'Harovinton' with the breeding line SQ97-0263_3-1a, which lacks the 7S α', 11S A, 11S A, 11S A and 11S A subunits. The storage protein composition of each individual in the F and F populations were profiled using SDS-PAGE. Based on the presence/absence of the subunits, genomic DNA bulks were formed among the F plants to identify genomic regions controlling the 7S α' and 11S protein subunits. By utilizing polymorphic SNPs between the bulks characterized with Illumina SoySNP50K iSelect BeadChips at targeted genomic regions, KASP assays were designed and used to map QTLs causing the loss of the subunits. Soybean storage protein QTLs were identified on Chromosome 3 (11S A), Chromosome 10 (7S α' and 11S A), and Chromosome 13 (11S A), which were also validated in the F RIL population. The results of this research could allow for the deployment of marker-assisted selection for desired storage protein subunits by screening breeding populations using the SNPs linked with the subunits of interest.

Seed Storage Proteins of Faba Bean (Vicia faba L): Current Status and Prospects for Genetic Improvement

“…In humans, dependence on poor quality proteins can result in reduced immunity and underdeveloped mental and physical capacity among young children 56 . Also, animal feeds deficient in critical amino acids can cost farmers in form of animal feed supplements of industrially synthesized S-AA 55 .…”

Genome-wide association study of seed protein, oil and amino acid contents in soybean from maturity groups I to IV

“…Unlike protein, only a few studies investigating the genetic control of variation in the amino acid content of soybean have been published (Panthee et al 2006 ; Vaughn et al 2014 ; Warrington et al 2015 ). However, many QTL related to the 7S (β-conglycinin) and 11S (glycinin) fractions of soybean storage proteins have been identified on Chrs 1, 3, 4, 6, 10, 13, 16, 17, 19 and 20 (Panthee et al 2004 ; Ma et al 2016 ; Boehm Jr. et al 2018 ). The glycinin fraction contains higher levels of S-containing amino acids than the β-conglycinin fraction (Warrington et al 2015 ).…”

Soybean β-conglycinin Induces Intestinal Immune Responses in Chicks

“…Soybean, a member of the legume's family, is a good protein source because of a high protein composition (37-42%) with a rich and good balance of amino acids as well as a nutritional and healthy food (Radder & Husen 2017). However, they have a high content of storage proteins that inhibit their nutrient function and can also cause inflammation and allergic reactions (Boehm et al 2017). It reduces the nutritional value of soybeans.…”