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The medical use of ozone has been based on its antibacterial and oxidative characteristics. Currently ozone is being discussed in dentistry as a possible alternative oral antiseptic agent. In this study, we examined the hemostatic effect of water and gel contains aqueous ozone in animal testing. The mean of bleeding time using ozonated water and ozonated gel were observed for significant difference compared with no treatment. 0.5 ppm diluted ozonated water shortened bleeding time the same as 4.0 ppm ozonated water. These results suggest that ozonated water and gel which contains aqueous ozone show hemostatic ability which is almost equal to the Bosmin solution and Liquid Thrombin.

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