BackgroundFor a long time, the Christie-Atkinson-Munch-Peterson (CAMP) test has been a standard test for the identification of Streptococcus agalactiae, and a positive result for S.agalactiae has been considered sensitive enough.MethodsTo confirm whether a positive CAMP test is a requirement for the identification of S.agalactiae, five suspected CAMP-negative S.agalactiae isolates from two hospitals, confirmed as Gram-positive and catalase-negative streptococci, were verified by the CAMP test in three batches of plates from two manufacturers and identified by the Phoenix system, MALDI-TOF MS, the PCR assay and the 16S rDNA gene sequencing.ResultsAll five suspected strains were S.agalactiae, four of which were CAMP-negative and one of which was not S.agalactiae by the PCR assay.ConclusionsA positive CAMP test was lacking sensitivity for the identification of S.agalactiae, and the question of whether the cfb gene is worthy of targeting should be further studied.
With the rapid development of industry in the new era, green, efficient energy utilization occupies an important position, thus posing new requirements for practical and innovative skills of undergraduate students. In this paper, we use metal-loaded ZSM-5 catalyzed MTA reaction as an industrial practice example to investigate the process and significance of students' design in practical innovation courses. The catalyst research example is used to create a training system for students' problem identification, practical process evaluation and other competency development processes, to explore the influence of subjective or objective aspects that may be encountered during the practical process, and to investigate the significance of industrialized practical experiments for students' competency development.
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