In this study, a lab-scale rotating biological contactor (RBC) treating a synthetic NH 4 ؉ wastewater devoid of organic carbon and showing high N losses was examined for several important physiological and microbial characteristics. The RBC biofilm removed 89% ؎ 5% of the influent N at the highest surface load of approximately 8.3 g of N m؊2 day ؊1 , with N 2 as the main end product. In batch tests, the RBC biomass showed good aerobic and anoxic ammonium oxidation (147.8 ؎ 7.6 and 76.5 ؎ 6.4 mg of NH 4 ؉ -N g of volatile suspended solids [VSS] ؊1 day ؊1 , respectively) and almost no nitrite oxidation (< 1 mg of N g of VSS ؊1 day ؊1 ). The diversity of aerobic ammonia-oxidizing bacteria (AAOB) and planctomycetes in the biofilm was characterized by cloning and sequencing of PCR-amplified partial 16S rRNA genes. Phylogenetic analysis of the clones revealed that the AAOB community was fairly homogeneous and was dominated by Nitrosomonas-like species. Close relatives of the known anaerobic ammonia-oxidizing bacterium (AnAOB) Kuenenia stuttgartiensis dominated the planctomycete community and were most probably responsible for anoxic ammonium oxidation in the RBC. Use of a less specific planctomycete primer set, not amplifying the AnAOB, showed a high diversity among other planctomycetes, with representatives of all known groups present in the biofilm. The spatial organization of the biofilm was characterized using fluorescence in situ hybridization (FISH) with confocal scanning laser microscopy (CSLM). The latter showed that AAOB occurred side by side with putative AnAOB (cells hybridizing with probe PLA46 and AMX820/KST1275) throughout the biofilm, while other planctomycetes hybridizing with probe PLA886 (not detecting the known AnAOB) were present as very conspicuous spherical structures. This study reveals that long-term operation of a lab-scale RBC on a synthetic NH 4 ؉ wastewater devoid of organic carbon yields a stable biofilm in which two bacterial groups, thought to be jointly responsible for the high autotrophic N removal, occur side by side throughout the biofilm.Sustainable wastewater treatment systems are being developed that minimize energy consumption, CO 2 emission, and sludge production. However, these systems typically yield effluents rich in ammonium-nitrogen (NH 4 ϩ -N) and poor in biodegradable organic carbon, thereby making them less suitable for biological N removal through the conventional nitrification-denitrification sequence.Different N removal processes that could be successfully integrated in a sustainable wastewater treatment system are being studied. The Sharon process (single-reactor high-activity ammonium removal over nitrite) (15) uses the principle that at higher temperatures (30 to 35°C), pH 7 to 8, and a cell residence time of 1 day, aerobic ammonia-oxidizing bacteria (AAOB) are able to maintain themselves in the system while nitrite-oxidizing bacteria (NOB) are washed out. Given the reaction stoichiometry of the two groups of nitrifying bacteria (equations 1 and 2), this proces...

Evaluations of soil organic carbon (SOC) stocks are often based on assigning a carbon density to each one of a number of ecosystems or soil classes considered, using data from soil profiles within these categories. A better approach, in which the use of classification methods by which extrapolation of SOC data to larger areas is avoided, can only be used if enough data are available at a sufficiently small scale. Over 190 000 SOC measurements (0–24 cm) have been made in the Flemish cropland (the Northern part of Belgium) in the 1989–2000 period. These SOC data were grouped into 3‐year periods and as means plus standard deviation per (part of) community (polygons). This large dataset was used to calculate SOC stocks and their evolution with time, without data extrapolation. Using a detailed soil map, larger spatial groups of polygons were created based on soil texture and spatial location. Linear regression analysis showed that in the entire study area, SOC stocks had decreased or at best had remained stable. In total, a yearly decrease of 354 kton OC yr−1 was calculated, which corresponds with a net CO2 emission of 1238 kton CO2 yr−1. Specific regions with a high carbon sequestration potential were identified, based on SOC losses during the 1989–2000 period and the mean 1999 SOC content, compared to the average SOC content of soils in Flanders with a similar soil texture. When restoring the SOC stocks to their 1990 level, we estimated the carbon sequestration potential of the Flemish cropland soils to be some 300 kton CO2 yr−1 at best, which corresponds to a 40‐year restoration period. In conclusion, we can say that in regions where agricultural production is very intense, carbon sequestration in the cropland may make only a very modest contribution to a country's effort to reduce greenhouse gas emissions.

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A procedure for start-up of oxygen-limited autotrophic nitrification-denitrification (OLAND) in a lab-scale rotating biological contactor (RBC) is presented. In this one-step process, NH 4 + is directly converted to N 2 without the need for an organic carbon source. The approach is based on a sequential addition of two types of easily available biocatalyst to the reactor during start-up: aerobic nitrifying and anaerobic, granular methanogenic sludge. The first is added as a source of aerobic ammonia-oxidizing bacteria (AAOB), the second as a possible source of planctomycetes including anaerobic ammonia-oxidizing bacteria (AnAOB). The initial nitrifying biofilm serves as a matrix for anaerobic cell incorporation. By subsequently imposing oxygen limitation, one can create an optimal environment for autotrophic N removal. In this way, N removal of about 250 mg of N L -1 d -1 was achieved after 100 d treating a synthetic NH 4 + -rich wastewater. By gradually imposing higher loads on the reactor, the N elimination could be increased to about 1.8 g of N L -1 d -1 at 250 d. The resulting microbial community was compared with that of the inocula using general bacterial and AAOB-and planctomycete-specific PCR primers. Subsequently, the RBC reactor was shown to treat a sludge digestor effluent under suboptimal and strongly varying conditions. The RBC biocatalyst was also submitted to complete absence of oxygen in a fixed-film bioreactor (FFBR) and proved able to remove NH 4 + with NO 2 -as electron acceptor (maximal 434 mg of NH 4 + -N (g of VSS) -1 d -1 on day 136). DGGE and real-time PCR analysis demonstrated that the RBC biofilm was dominated by members of the genus Nitrosomonas and close relatives of Kuenenia stuttgartiensis, a known AnAOB. The latter was enriched during FFBR operation, but AAOB were still present and the ratio planctomycetes/ AAOB rRNA gene copies was about 4.3 after 136 d of reactor operation. Whether this relates to an active role of AAOB in the anoxic N removal process remains to be solved.

-Different European Biomass Expansion Factors (BEFs) were compared for the inventory-based quantification of total aboveground and belowground biomass in forests. Therefore a qualitative analysis is performed on the biomass results obtained through the BEF approach and those from experimentally established allometric relations based on destructively sampled and fully excavated trees. Total organic carbon (OC) stock in aboveground and belowground living biomass of Flemish forests amounts to 12 Mt on average, with a significantly larger OC stock per hectare in deciduous forests compared to coniferous or mixed forest types. Total forest biomass seems to be fairly well approximated by a multiplication of the standing stock with either one of the applied BEFs. However an indication of the volume and age class for which the BEFs are established and a refined diameter-volume-biomass relation for oak trees in Europe, are required to gain more accurate results.

Because of the large spatial and temporal variability of soil organic carbon (SOC) dynamics, a modelling approach is crucial in detailed regional analyses. Several estimates of regional scale SOC sequestration potential have been made using dynamic soil organic matter (SOM) models which have been linked to spatial databases contained within a Geographic Information System. In all these previous studies, a large‐scale model validation, which provides information on the general model performance for the study area under concern, was impossible because of lack of data. A data set of over 190 000 SOC measurements, grouped as means per community and covering the period 1989–2000 was available for Flanders in northern Belgium. In order to validate the DNDC model at a large spatial scale, we used this data set along with detailed pH, soil texture and crop areas which were all available at the municipality scale to simulate SOC stocks for the entire study area during the period 1990–2000. A minor adjustment of the initial distribution of SOC in the model's SOC pool was necessary to fit the simulated SOC stock changes to the measured decrease of −475 kg OC ha−1 year−1 (0–30 cm). Although DNDC was able to simulate the SOC stock changes well for the whole study area, the simulated decrease in the SOC stocks was overestimated for communities predominantly having sandy textures and underestimated for communities with silt loam to silt textures. This study also urges caution with the application of SOM models at regional scales after limited validation or calibration at the field scale as these do not guarantee good simulation of spatial variation in SOC changes.

Total soil organic-carbon (SOC) stocks for grassland soils in Flanders (N Belgium) were determined for the Kyoto Protocol reference year 1990 and 2000 in order to investigate whether these soils have been CO 2 sinks or sources during that period. The stocks were calculated by means of detailed SOC datasets, which were available at the community scale for the whole of Flanders. The total SOC stocks for Flemish grassland soils (1 m depth) were estimated at 38 Mt SOC in 1990 and 34 Mt SOC in 2000. The loss of SOC resulted from a decrease in the SOC content of grassland soils (71%) and could also partly (29%) be explained by a decline in grassland area. Significant decreases in %SOC for the 0-6 cm depth layer were found for the 1990s for the coarser-textured soils with SOC losses ranging between -0.3% and -0.5% over the 10 y period. Specific management practices that disturb the SOC balance such as conversion to temporary grassland and a reduction of animal-manure application are hypothesized to have contributed to the observed loss of SOC stocks. We furthermore conducted an analysis of uncertainty of the 1990 and 2000 grassland SOC-stocks calculation using Monte Carlo analysis. Probability-distribution functions were determined for each of the inputs of the SOC-stock calculation, enabling us to assess the uncertainty on the 1990 and 2000 SOC stocks. The frequency distributions of these simulated stocks both closely approached lognormal distributions, and their 95%-confidence intervals ranged between 150% and 50% of the calculated mean SOC stock. The standard error on the measured decrease in SOC stocks in Flemish grassland soils during the 1990s was calculated to be 7-8 Tg SOC, which is equivalent to twice this decrease. This clearly shows that large-scale changes in SOC stocks are uncertainty-ridden, even when they are based on detailed datasets.

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