A genomic clone encoding the gamma-kafirin gene from sorghum was isolated and sequenced. A 2938 bp sequenced fragment includes an intronless open reading frame of 636 nucleotides encoding a putative polypeptide of 212 amino acids. Comparison of the deduced amino acid sequence of gamma-kafirin with the published sequences of gamma-prolamins of maize, and Coix revealed highly conserved domains. The N-terminal region of these proteins contains the conserved hexapeptide PPPVHL, which is repeated eight times in gamma-zein, four times in gamma-kafirin and three times in gamma-coixin. The number of PPPVHL repeats accounts predominantly for the differences in the molecular weights of gamma-prolamins. Several putative regulatory sequences common to the gamma-kafirin and gamma-zein genes were identified in both the 5' and the 3' flanking regions. Putative GCN4-like regulatory sequences were found at positions -192 and -476 in the 5' flanking region of gamma-kafirin. In the 3' noncoding region, three putative polyadenylation signals, two AATAAT and one AATGAA, were found at positions +658, +716, and +785, respectively. In order to investigate the role of the putative GCN4-like motifs and other possible cis-acting element(s) of the gamma-kafirin promoter, a series of deleted and chimeric promoter constructs were introduced into maize, Coix and sorghum tissues by particle bombardment. Histochemical analysis of beta-glucuronidase (GUS) activity in different tissues indicated that the element(s) responsible for tissue specificity is probably located in the 285-bp proximal region of the promoter, while the remaining promoter sequence seems to carry the element(s) responsible for the quantitative response.
We hope that you enjoy REEUSP's new layout. We are always striving to improve our journal so that, besides the quality articles you are familiar with, you may have a pleasurable read in an aesthetically pleasing package. Our first issue of the year was published with a green cover (signaling the beginning of each new year), the second with a yellow cover (bringing to light interesting issues), the third and fourth will also have their own colors in order to identify each quarter, making it easier for you to find them on your library shelves. Another piece of good news is that our journal is now indexed in another database: CUIDEN, the well-respected Spanish language database of the Fundação Index (Index Foundation). It includes scientific research from Spanish and Iberian-American nursing, containing both clinical care with all its specialties and different methodological, historical, social and cultural focuses. It contains journals, books, monographs and unpublished material. The content of CUIDEN is published in an annual series in the Revista Index de Enfermería (Nursing Journal Index). Professionals concerned with disseminating the results of their research domestically and internationally will now have an opportunity to achieve this objective through Spain, a country whose language is similar to Portuguese. And besides being one of the major European languages, it is also the official language for Latin American countries. In this quarter's journal, concern regarding the education of nurses is presented in an article that discusses the challenges that exist for educators in nursing. It describes how competencies should be developed for intervention in the health-disease process for the Brazilian population. There is also a reflection on the importance of critical thinking and diagnostic accuracy in nursing by a North American nurse. Social representations of child care workers from three Sao Paulo public university day care centers, concerning the care of children, emerges in research that addresses what kind of contribution nurses can make regarding care and education, an inherent role for these workers. Mental health is at issue in an article that reflects upon citizenship and quality of life for those with psychiatric disorders, describing the challenges to be overcome. Still another study addresses how family members of psychiatric patients are included or excluded from treatment. Further still is research that characterizes the care given to hospitalized women in a psychiatric clinic in Rio Grande do Norte after their first hospitalization, embarking on what the authors, our colleagues, characterize as the women's "psychiatric career." The suffering of women who have abortions is described in an article that details what it means for those who go through this process. The treatment given to hospitalized women emerges in the research that exposes the social representations of parturient women and their group lodging experience in a teaching hospital. Also included in this quarter's issue is: Research fr...
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