Decline of species population, low natural regeneration, and heavy competition on field sites require the planting of large seedling stocks to restore Taxus chinensis and Phoebe chekiangensis in tropical China. In this study, we examined the effects of different formulations and application rates of controlled-release fertilizers (CRF) of nitrogen (N) and phosphorus (P) on nursery seedling growth and nutritional attributes. The objective was to determine optimum formulation (N:P2O5 ratio) and application rate to increase nutrient reserves of the seedlings before transplanting to the field. Four formulations (17-9-13 to 19-6-14 N-P2O5-K2O ratios) and four application rates (1.5 kg m -3 to 4.5 kg m -3 ) were used in a double-factors factorial design with 3 replications. The results showed that CRF formulation can affect nutritional attributes, while application rate modified seedling growth and nutritional attributes. The optimum seedling response occurred with the 17-6-16 formulation at the rate of 3.5kg m -3 . These findings will guide nursery practice in the production of high-quality seedlings for optimum survival and growth in the field. (2019). Responses of Taxus chinensis and Phoebe chekiangensis seedlings to controlledrelease fertilizer in various formulations and application rates. iForest 12: 254-261.