In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.

This study examines the relationship between leaders’ humility and their career success. We propose that humble leaders are more likely to occupy central positions in their subordinate teams’ voice networks where they improve their own performance and gain favorable reward recommendations. We also argue that in seemingly disadvantageous competitive work contexts, humble leaders become more central in the team voice network and increase their career prospects. We found support for these hypotheses in a multisource field study of 116 supervisors, 461 subordinates, and 34 shop managers from a Chinese company and in a vignette-based experiment with 233 working adults. Theoretical and practical implications for career success, leader humility, and voice literature are discussed.

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