Laboratory tests to identify the viral genome, the antigen, or specific antibodies are used for a definitive diagnosis of avian metapneumovirus (AMPV) infection. Methods that can be used for antibody determination are the virus neutralization test and the enzyme-linked immunosorbent assay. To monitor the presence of AMPV in Korean chickens, sera were taken from chickens in 20 flocks. Of the 197 serum samples tested by enzyme-linked immunosorbent assay, 144 (73.1%) were positive for AMPV. The incidence of AMPV antibodies was higher in broilers (97.3%; 36 of 37 chickens) than in layers (67.5%; 108 of 160 chickens). Age was a factor, with very few positive samples obtained from young birds and the highest positive rates obtained for birds more than 100 d old.
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