Olanzapine and sertindole were less efficacious dopamine antagonists in intact cell assays, possibly due to avid uptake in cells. For sertindole, the weak hD2S receptor antagonism in intact cells corresponded to a weak in vivo central dopamine antagonism assessed in rats. However, for olanzapine, hD2S receptor binding affinity correlated better with its in vivo dopamine antagonist potency. Such discrepancies may be further explained by relative differences of the compounds in penetrating into the brain.

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Thyrotrophin in individual pituitaries obtained from fetal and prepubertal pigs was quantified by homologous radioimmunoassay (RIA) and heterologous radioreceptor assay (RRA). Relative evolution of pituitary TSH contents and concentrations with age were in good agreement as measured by both assay systems although the quantity of TSH detected by RRA appeared consistently lower than that measured by RIA. Thyrotrophin was first detected in pituitaries of fetal pigs at day 75 of gestation. Thereafter the pituitary content of TSH increased to approximately 45 micrograms/pituitary in the oldest group tested (6 weeks of age). The pituitary TSH concentration rose sharply until birth (114 +/- 1 day post coitum) and thereafter remained increased at a concentration of approximately 400 ng/mg wet weight.

A solid-phase enzymeimmunoassay (EIA) was developed for progesterone, based on horse radish peroxidase as the enzyme-label and Dasp (sheep anti-rabbit Ig, covalently linked to cellulose) for separation of antibody-bound and free hormone. The validity of the assay was substantiated in accordance with criteria for sensitivity, precision, accuracy and specificity; it is simple to perform and can be done in one day. Milk samples were taken on the day of A.I. (day 0) and on day 21 from 60 dairy cows which belonged to an artificial insemination (A.I.) station in the central part of Thailand. Conception rate (C.R.) after first A.I., as diagnosed by the milk-progesterone test, was 31.7 per cent. Based on rectal exploration on day 84-93 and on 'non-return' at 85 days. C.R. was 20.0 per cent and 23.3 per cent, respectively. All animals except one showed non-luteal phase progesterone levels at the time of A.I. It is concluded that low C.R. in these cows is not due to inseminations during the luteal phase of the oestrous cycle.