Two Clostridium thermocellum strains were improved for ethanol tolerance, to 5% (v/v), by gradual adaptation and mutation. The best mutant gave an ethanol yield of 0.37 g/g substrate, with a growth yield 1.5 times more than its parent. Accumulation of acids and reducing sugars by the mutant strain with 5% (v/v) ethanol was lower than that of the parent strain with 1.5% (v/v) ethanol.
A rapid RP-HPLC method was developed and validated for simultaneous estimation of metformin, glimepiride and pioglitazone from pharmaceutical dosage forms. A sensitive chromatographic separation was accomplished on C18 column (100 × 4.6 mm, 5 µ) with mobile phase consisting of Methanol: phosphate buffer (pH 3.6 adjusted with ortho phosphoric acid) in the ratio of 75:25 v/v, at a flow rate of 1.0 ml / min and monitored at 238 nm. The developed method was validated in terms of accuracy, precision, linearity and limit of detection, limit of quantification, robustness and solution stability. The proposed method can be used for the routine estimation of these drugs in combined pharmaceutical dosage forms
COVID19 is an infectious disease caused by a new coronavirus introduced to humans for the first time. It spreads from person to person mainly through the droplets produced when an infected person speaks, coughs or sneezes. Older adults and people who have certain underlying conditions like heart or lung disease or diabetes are at increased risk of severe illness from COVID-19 illness. Healthcare systems could be overwhelmed treating both patients with flu and patients with COVID-19. This means getting a flu vaccine during 2020-2021 is more important than ever. While getting a flu vaccine will not protect against COVID-19 there are many important benefits, such as:1 Flu vaccines have been shown to reduce the risk of flu illness, hospitalization, and death. Getting a flu vaccine can also save healthcare resources for the care of patients with COVID-19. Confirmed COVID-19 cases worldwide have exceeded 76 million, with over 1.6 million fatalities and more than 42 million recoveries, according to the latest tally from Johns Hopkins University.
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