The roots of Margaretta rosea Oliv., subsp. rosea Bullock contain cardenolides mixed with Kedde negative ester glycosides. 6 Cardenolides were identified by paper chromatography (PC) and thin layer chromatography (TLC). Three of them were free aglycones (uzarigenin (l), corotoxigenin (3) and coroglaucigenin (5)' the other three their corresponding 6-deoxy-p-~-allopyranosides (ascleposide (2), gofruside (4) and frugoside (6). Only two cardenolides (1 and 5) were isolated in crystalline form. The behaviour of the more polar portion of the glycoside mixture shows that D-gluco derivatives of a t least 5 of the 6 cardenolides mentioned were also present.The Kedde negative glycosides were a complicated mixture containing mainly 2,6-dideoxysugars linked to esterified polyhydroxy-pregnanes. After mild acid hydrolysis cymarose, oleandrose
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