The purpose of this study was to examine the various sulphur (S) fractions in experimental pot calcareous soil treated with Bio sulphur granules (BSG) in order to assess the impact of granular sulphur fertilization in S deficient calcareous soil using blackgram (Var. VBN-8) as a test crop.Factorial randomized block design with ten treatments (T1- Absolute control;T2-Recommended dose of NPK and S (Control);T3-Soil test based NPK; T4-T3 + S as Elemental Sulphur @ 40 kg S/ha; T5-T3 + S as BSGI@ 40 kg S/ha; T6-T3 + S as BSGII@ 40 kg S/ha;T7-T3 + Vermicompost @ 4 t ha-1; T8-T4 + Vermicompost @ 4 t ha-1;T9- T5 + Vermicompost @ 4 t ha-1; T10- T6+ Vermicompost @ 4 t ha-1 ) replicated thrice and 5 pots were maintained for each replication. The results of this study revealed that there was an upward trend in all S fractions in every treatment (T1 to T10), in the following order: organic > inorganic > water soluble > exchangeable S. The pot that received vermicompost coupled with BSG II (T10) (ES@ 40 kg ha-1 and MethylobacteriumthiocyanatumVRI7-A4 as S source) was found to have the greatest S-fraction and was higher than other treatments. Therefore, using BSG II in conjunction with vermicompost is necessary to preserve the availability of S nutrients in calcareous soil and increase the solubility of nutrients through S-oxidation.
Background: Sulphur (S) is an essential macronutrient required for growth and development of plants. This study was carried out to investigate the effect of Bio Sulphur Granules (BSG) developed with elemental sulphur and sulphur oxidizing bacteria (SOB) on the antioxidative defense system in blackgram under sulphur deficient calcareous soil. Methods: Bio Sulphur Granule (BSG) was developed using ES plus SOB and its efficacy was tested in a pot experiment with blackgram as a test crop from April to June 2022. Plant samples were collected and analyzed in the laboratory for anti-oxidant status of blackgram, how sulphate stress affects the physiological and metabolic processes of plants, which in- turn affects crop yield. Results: Photo assimilatory pigments were decreased and carbohydrates (sugar and starch) were accumulated in leaves of no sulphur treated plants. Hydrogen peroxide in without S supplied plant caused oxidative damage in plants, which also evident by the increase in activity of super oxide dismutase, catalase, peroxidase and ascorbate. The findings showed that by applying S as BSG granules (ES @ 40 kg S ha-1 + Methylobacterium thiocyanatum VRI7-A4) to S-deficient calcareous vertisols might prevent the oxidative damage of plant cells there by improve the growth of blackgram by S-oxidation.
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