Mud loach, Misgurnus wizolepis (Gunther), were sex reversed using the immersion technique. Fry were raised in water containing 50, 100 or 200 |ig oestradiol-17P 1"^ for 1, 2 or 3 weeks. Survival rates decreased as dose and duration of treatment increased: in most instances they were significantly (P < 0.05) lower than those of the controls. Growth rates were unaffected except for fish in the 200 ^ig r^ treatments. The 200 (Xg T^ treatments for 2 and 3 weeks produced monosex female populations. The 100 ng 1~^ treatment for 3 weeks produced 99% females and 1% intersex individuals. Histologically, ovaries of sex-reversed fish were similar to those of normal females. Morphologically, however, they were different in that the ovaries of sex-reversed flsh were not connected to the genital pore. The hormone treatments affected the size and morphology of the pectoral Uns, which exhibit sexual dimorphism in mud loach.
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