The classic enzyme-linked antiglobulin test (ELAT) used to detect and quantify
the amount of IgG antibodies on red blood cells (RBC) is sensitive to hemolysis and erythrocyte
enzymatic activities. We describe a new ELAT by using glucose oxidase (GO) linked
to antihuman IgG. The optical density base line of GO-ELAT, alkaline phosphatase-ELAT
and peroxidase-ELAT were, respectively, 0.180, 0.350 and 0.550. This very low baseline of
GO-ELAT was due to the absence of hemolysis (the pH of the GO substrate is 6.5). This
technique is ten times more sensitive than the indirect antiglobulin test and detects up to
1 ng/ml of anti-D alloantibodies. Additional advantages of the technique are (1) there is no
intrinsic GO enzyme in RBC, and (2) it is not necessary to fix the RBC.
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