A reverse~phase high-performance liquid chromatography method was developed for the simultaneous separation and quantitation of tocopherols, tocotrienols and oryzanols present in rice bran oil. Tocopherols and tocotrienols were quantitated by fluorescence detection and oryzanols (ferulic acid esters of sterols and triterpene alcohols) by photodiode array detection. Chemical ionization mass spectrometry was used to identify cycloartenyl ferulate, 24-methylene cycloartanyl ferulate, campesteryl ferulate, /Nsitosteryl ferulate and cycloartanyl ferulate as the major oryzanols separated by this procedure. The levels of these nutritionally significant components were found to vary in fully processed, edible rice bran oils from different manufacturers.

The seeds of nineHerrania and nineTheobroma species were surveyed for fatty acid, sterol, tocopherol and tocotrienol compositions. Principal component and cluster analyses suggested that these analytes could be used collectively as chemotaxonomic criteria to differentiate theHerrania species from theTheobroma species, as well as to provide subgroup distinctions within each genus for comparison to the existing classification schemes.

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