Following ultra‐violet irradiation of the hydrocarbon‐utilizing yeast, Saccharomycopsis lipolytica, a number of mutant strains were isolated which failed to show the normal staining reaction with iodine. Exponential phase cells of the mutant strains were found to contain less carbohydrate and more crude protein than wild type cells in the case of both glucose‐grown and n‐alkane‐grown cultures. The difference between wild type and mutant carbohydrate levels was greater for glucose‐grown than for n‐alkane‐grown cells. Carbohydrate fractionation revealed that the mutant cells were deficient in glycogen, particularly the acid‐soluble fraction.
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