The design and construction of an analyser for determining glucose concentrations in solution are described. The dissolved p-D-glucose reacts with oxygen in the presence of glucose oxidase and the maximum rate of oxygen uptake, which is proportional t o the initial concentration of glucose, is measured using an oxygensensing probe. The output of the probe is processed electronically and the glucose concentration is displayed on a meter. The analyser has a linear response for aqueous solutions containing up to 500 mg per 100 m1 of glucose. The performance of the analyser is compered directly with that of a Technicon Mark I auto analyser. For serum and plasma samples a correlation coefficient of 0.992 is obtained and using whole blood, treated with sodium nitrite, the correlation coefficient is 0.940. Since only 10 p1 samples are used and plasma samples can be analysed at a rate of one complete sample per minute, this analyser may be conveniently used in a diabetic clinic.
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