In a majority of cases, PUUV infection results in thrombocytopenic AKI. Fever is a requirement for diagnosis, while elevated LDH and CRP values are also frequently observed. Overall, early renal outcomes were excellent.
Summary. Eight normal subjects underwent two intravenousglucose tolerance tests to determine the between and within subject variation of the first phase insulin response. Variability was represented by the coefficient of variation. The between subject variation for the incremental 0-10 rain insulin area was 58%, and the within subject variation was 22% (median value), range 3-55%. The variation of the first phase response expressed in four different ways was compared. The total and incremental (above fasting levels) 0-10 rain areas provided less variable results (variation 52 and 58%) than the l + 3 rain insulin levels (variation 72%) or mean of the incremental 3-5 rain insulin levels (variation 66%). The ratio of the incremental 0-10 min insulin to glucose areas was as variable (variation 53%) as the insulin responses alone. The variability of insulin responses to intravenous glucose severely limits their value as early predictors of B-cell failure.
Key words: Variability, insulin, glucose.Loss of the first phase insulin response to intravenous glucose is the earliest detectable metabolic abnormality in subjects who later develop Type 1 (insulin-dependent) diabetes [1], and the intravenous glucose tolerance test (IVGTT) is used to complement immunological markers [2] in identifying individuals at risk.The insulin response to the IVGTT is already known to be highly variable [3], but we have concentrated on variability of the first phase insulin response, since this appears to have the greatest prognostic value [I]. We have investigated the between and within subject variation of the first phase insulin response because these seem crucial for interpretation of cross-sectional or longitudinal data.
An optimized method is described for the mass fragmentographic determination of uniformly labelled (13C)glucose in human plasma using a butylboronic acid acetate derivative, and capillary gas chromatography. The advantages of the method are the ease and speed of the derivatization procedure, the small sample size, high precision (interassay coefficient of variation 5.7%), and applicability of a relatively low-cost mass spectrometer. This method allows glucose tracer experiments to be performed in man using the bolus injection technique, necessitating analysis of many samples. The results on glucose turnover obtained in a clinical experiment were in full agreement with previously published data.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.