This paper presents the synthesis and physico-chemical characterization of iron(II) gluconate as a potential antianemic drug for oral administration. Iron(II) gluconate was synthesized from sodium gluconate and iron(II) sulphate as precursors. The reaction product was purified by treating the crude reaction mixture with the acidic and basic ion exchange resins for the removal of Na + i SO4 2ions. Iron(II) gluconate with the purity greater than 90% was obtained after evaporation in a vacuum evaporator, the treatment with aprotic solvent and drying in a desiccator over a dehydration agent. Molar ratio of 1:2 of iron and gluconate remains was determined by applying the Job's method. Structural properties of the obtained iron(II) gluconate were determined by using UV-VIS spectrophotometry, infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1 H-NMR) and X-ray diffraction (XRD) methods. Thermal degradation of iron(II) gluconate was monitored by differential scanning calorimetry (DSC) and the results showed that synthetized and purified Iron(II)-gluconate was most probably in the form of dihydrate. The qualitative and quantitative analysis of iron(II) gluconate was performed by high performance liquid chromatography (HPLC).
The aim of this work is the protection of piroxicam from photodegradation by forming inclusion complex with 2-hydroxypropyl-β-cyclodextrin. Piroxicam:2-hydroxypropyl-βcyclodextrin molecular inclusion complex was prepared by coprecipitation method with 1:1 molar ratio. Structural characterization of the complex, the corresponding physical mixture and complexing agents of piroxicam and 2-hydroxypropyl-β-cyclodextrin was carried out by X-ray diffraction (XRD), proton nuclear magnetic resonance (1 H-NMR) and Fourier transform infrared spectroscopy (FTIR). Photosensitivity to daylight of piroxicam and piroxicam:2-hydroxypropyl-β-cyclodextrin inclusion complex was investigated by FTIR. The investigations show that higher photostability of piroxicam was achieved in the complex than in non-complexed piroxicam.
Solanidine (C17H43O/V) is a steroidal aglycone of glycoalkaloids and an important precursor for the synthesis of hormones and some pharmacologically active compounds. Glycoalkaloids are hydrolysed by mineral acid yielding solanidine and a carbohydrate moiety. In this paper the kinetics of hydrolysis of glycoalkaloids from potato (Solanum tuberosum L) tuber sprouts by using solid-liquid systems were studied as well as solanidine isolation from the liquid phase of the system. The dried and milled tuber sprouts of potato were used as the solid phase and solutions of hydrochloric acid of different concentration in 96 % vol. ethanol, mixed with chloroform in a volume ratio of 2:3, 1:1, 3:2 and 4:1, were used as the liquid phase. The aim of the paper was to choose the optimal concentration of hydrochloric acid in ethanol, the volume ratio of hydrochloric acid in ethanol to chloroform in the liquid phase and the time for solanidine hydrolytic extraction, as well as to isolate solanidine from the liquid phase
UV-VIS spectrophotometric investigations of Cu(II) complexes with hydroge-nated dextran showed that the complexation of Cu(II)-ions began at pH > 7. The formation of Cu(II) complexes with dextran monomer units was observed at pH 7-12. With further increase in solution pH > 12, the Cu(II)-dextran complex decomposed to Cu(OH)42~-ions and dextran. With increasing solution pH the absorption maximum of complex solutions increased and shifted to shorter wavelength (hypsochromic shift) compared with uncomplexed Cu(II). The UV spectra displayed bathochromic shifts. The changes of UV-VIS spectra with increasing in solution pH confirmed the formation of different kinds of complex species. The correlation between the results of UV-VIS spectrophotometry and the central metal ionligand coordination predicted that the copper binding within the complex depended on the pH and participation H2O molecules. Dextran complexes with Cu(II) were formed by the displacement of water molecules from the coordination sphere of copper by OH groups. The analysis indicated that the Cu(II) center was coordinated to two glucopyranose units of dextran. The spectrophotometric parameters of the investigated complexes were characteristic of a Cu(II)-ion in a square-planar or tetragon ally distorted octahedral coordination
In order to develop a method for rapid, efficient and accurate detection and quantitative determination of gliadin proteins from wheat flour, the usage of different solvents and different separation conditions were investigated by reversed-phase high performance liquid chromatography (RP-HPLC). After albumin and globulins were removed from wheat flour (100 mg) with a salt solution, the gliadin extraction was achieved with ethanol, 1-propanol and isopropanol (50% v/v, 60% v/v and 70% v/v each). Then, the glutenin fractions were extracted using nitrogen and 50% (v/v) aqueous solution of 1-propanol containing Tris-HCl (0.05 mol/dm 3 , pH = 7.5) at 60 °C, urea (2 mol/dm 3) and dithioerythritol (1%). The separation and quantitative gliadin determination were carried out by RP-HPLC chromatography on C3 column maintained at different temperatures: 40 °C, 45 °C and 50 °C. In order to determine the absolute amounts of the protein type, gliadin protein standard was used. The obtained results in terms of repeatability, linearity and accuracy showed that RP-HPLC chromatography could be used as a fast, relatively simple and reliable way to quantify all types of gluten proteins in wheat flour and can be applied to assess the quality of cereals as raw materials or in cereal products.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.