Genetically engineered bacteria can be used for a wide range of applications, from monitoring environmental toxins to studying complex communication networks in the human digestive system. Although great strides have been made in studying single strains of bacteria in well-controlled microfluidic environments, there remains a need for tools to reliably control and measure communication between multiple discrete bacterial populations. Stable long-term experiments (e.g., days) with controlled population sizes and regulated input (e.g., concentration) and output measurements can reveal fundamental limits of cell-to-cell communication. In this work, we developed a microfluidic platform that utilizes a porous monolith to reliably and stably partition adjacent strains of bacteria while allowing molecular communication between them for several days. We measured small molecule production by the bacterial populations in response to stimuli using analytical chemistry methods and measured fluorescent output. The results are compared with communication and diffusion delay models. This porous monolith microfluidic system enables bacterial cell-to-cell communication assays with dynamic control of inputs, relatively long-term experimentation with no cross contamination, and stable bacterial population size. This system can serve as a valuable tool in understanding bacterial communication and improving biosensor design capabilities.
Prevalence of stroke in India is 90-222/100000 population. It causes so much morbidity to patient and family. Several classes of drugs are effective in preventing stroke recurrence by modifying the risk factors. There is limited data on long-term use of secondary prevention medications following stroke. Persistence is dened as the continuation of all medications patient was last prescribed at the end of 3 months. Adherence is dened as taking modied medications as per needs as per doctor's advice. A Methodology: ll patients with stroke both is chaemic and haemorrhagic and age more than 18 years , sustained in the one year when study is conducted as from the records of neurology department and attending the department for follow up are taken for study. A semi structured interview is done with them or their care givers to get information on their background characteristics, aetiology of stroke, comorbidities, cost of medication and affordability, various classes of medications administered and whether they are persistent in taking drugs, are they adherent to drugs, if not adherent/persistent ,reason for the same by open ended questions. A total of 260 patients were included, mean age was 58.5 years. Result: 78.5% had 3-month persistence and 3 month adherence 35.6%. Independent predictors of 3 month medication persistence included age more than 60 years, marital status, fewer medications prescribed at discharge and patients taking anti hypertensives . Most common reason for non-persistence was because the patients did not know why medication is being taken by them. Various factors play a role in persistence and adherenc Conclusion: e of medications in stroke. A multifactorial intervention including patient and health care providers are warranted, because of morbidity and mortality associated with every episode of stroke.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.