The hormonal response to LHRH and TRH was evaluated in three groups of male diaetics. Five patients were receiving therapy with the hypoglycemic agent glibenclamide, five were on NPH insulin and five were on dietary therapy alone. When compared to controls, the latter two groups had intact gonadotropin responses to LHRH. Despite normal basal gonadotropin levels, however, the group receiving glibenclamide therapy showed significantly exaggerated LH and FSH responses to LHRH. Both basal PRL and TSH levels, as well as the responses to TRH were normal in all three groups. These results indicate that LH, FSH, TSH and PRL secretion is intact in uncomplicated diabetes mellitus. The exaggerated LH and FSH responses to LHRH in the glibenclamide treated subjects are probably related to primary gonadal involvement; alternatively, there may be augmented pituitary gonadotropin secretion in this group.
Extracts of protozoa contain materials that resemble guinea pig insulin, which is noted for its unusual structure and properties. The protozoan derived materials react in the radioimmunoassay for guinea pig insulin; some but not all of these immunoreactive materials migrate on gel filtration in the position of authentic guinea pig insulin. Experiments were done to exclude artifacts in the assay as well as inadvertent contamination by guinea pig insulin. By immunological methods, we segregated the guinea pig type immunoactivity from that which has rat/pork type immunoactivity. These findings extend our studies of extracts of guinea pig tissues which also have these two types of insulin immunoactivities.
Repeated measurements of urine flow, every 10 min, and plasma ADH levels, every 20 min. were done in 7 healthy, normal young adults who consumed constant amounts of fluids and urinated voluntarily, and in 6 supine catheterized patients. Normal subjects who were hyperhydrated, stood up every 10 min. to urinate and then returned to a semirecumbant position. Catheterized patients who were also hyperhydrated assumed similar test procedure excluding postural changes. Variance spectra revealed significant ultraradian rhythms of about 100 ± 40 min/cycle in the flow of urine, in urine osmolality, sodium and potassium excretion as well as in plasma ADH levels, in normals, but all these rhythms were neither correlated nor phase locked to rhythms in ADH levels. In the supine patients, significant ultradian rhythms in urinary osmolality, sodium and potassium excretion were observed, but not in the flow of urine that fluctuated rapidly. Plasma ADH levels were constant, ranging from 0.5 to 1.5 pg/ml, without evidence for pulsatile episodic secretion. The finding that despite the hyperhydrated state plasma ADH levels in normal controls ranged between 0.5 to 15.0 pg/ml, suggests that in humans Downloaded by: East Carolina University. Copyrighted material.Rhythms in Urine Flow and ADH 67 orthostasis is a powerful stimulus for ADH secretion. It is also suggested that there are different control mechanisms responsible for urine flow and solute excretion.
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