Ether extracts of smoke condensates ("liquid smokes") from 20 different woods were screened for antimicrobial activity. Testing was done in trypticase soy broth of pH values in the range of 5.78-6.81 against three strains each of Aeromonns hydrophilu (ATCC 7965, K1414, BW37) and Sfaphylococcus aureus (ATCC 12600, LP, S6) incubated at 35°C. There was variation in inhibition of growth among types of liquid smoke, species, and strains of microorganisms tested. Staphylococcus uureus was generally more sensitive to inhibition than A. hydrophilu strains. The most inhibitory liquid smoke was derived from the sapwood of douglas fir. Other inhibitory liquid smokes were from birch, southern yellow pine, and aspen. The least inhibitory liquid smokes were derived from Iodge pole pine and mesquite woods.
The potential of waste fluids from clean-up of a meat grinder to support growth (8'C and 35°C) of artificially inoculated Lisferiu monocyfogenes Scott A was examined. Estimated generation times were 10.1 and 2.3 hr at 8°C and 8.9 and 2.2 hr at 35°C for a fluid collected during rinsing of the meat grinder and a fluid collected from the floor drain, respectively. A sanitizer fluid (25 ppm titratable iodine), collected after sanitation of the grinder, inactivated the inoculated cells. Thus, waste fluids may support growth of L. monocyrogenes in meat processing facilities, but frequent sanitation should inactivate the pathogen.
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