Highlights d Multi-omic maps of embryonic stem cells transitioning from naive to primed pluripotency d Phosphoproteome dynamics precede changes to epigenome, transcriptome, and proteome d ERK signaling is dispensable beyond the initial phase of exit from naive pluripotency d Comparative analysis of mouse and human naive and primed pluripotent states

Abstract-Group work is widespread in education. The growing use of online tools supporting group work generates huge amounts of data. We aim to exploit this data to support mirroring: presenting useful high-level views of information about the group, together with desired patterns characterizing the behaviour of strong groups. The goal is to enable the groups and their facilitators to see relevant aspects of the group's operation and provide feedback if these are more likely to be associated with positive or negative outcomes and where the problems are. We explore how useful mirror information can be extracted via a theory-driven approach and a range of clustering and sequential pattern mining. The context is a senior software development project where students use the collaboration tool TRAC. We extract patterns distinguishing the better from the weaker groups and get insights in the success factors. The results point to the importance of leadership and group interaction, and give promising indications if they are occurring. Patterns indicating good individual practices were also identified. We found that some key measures can be mined from early data. The results are promising for advising groups at the start and early identification of effective and poor practices, in time for remediation.

Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem-cell states. The two states that bookend the pluripotency continuum, naïve and primed, are well characterized, but our understanding of the intermediate states and transitions between them remain incomplete. Here, we dissect the dynamics of pluripotent state transitions underlying pre-to post-implantation epiblast differentiation. Through integrative analysis of the proteome, phosphoproteome, transcriptome, and epigenome of embryonic stem cells transitioning from naïve to primed pluripotency, we show that rapid, acute, and widespread changes to the phosphoproteome precede ordered changes to the epigenome, transcriptome, and proteome. Reconstruction of kinase-substrate networks reveals signaling cascades, dynamics, and crosstalk. Distinct waves of global proteomic changes mark discrete phases of pluripotency, characterized by cell surface markers that track pluripotent state transitions. Our data provide new insights into the multi-layered control of the phased progression of pluripotency and a foundation for modeling mechanisms regulating pluripotent state transitions. HIGHLIGHTS • Multi-ome maps of cells transitioning from naïve to primed pluripotency • Phosphoproteome dynamics precede changes to epigenome, transcriptome, and proteome • Kinase-substrate network reconstruction uncovers signaling dynamics and crosstalk • Proteins and cell surface markers that track pluripotent state transitions • Comparative analysis of mouse and human pluripotent states

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