Nanomaterial is defined as a material with the size range of 1 nm to 100 nm. Nanomaterials have landed in many sectors due to its enormous characteristics features. Nanoparticles are synthesized by chemical, physical and biological method in general. The chemical synthesis process comprises limitations like high production cost, usage of toxic chemicals, toxic by-product emission etc., Green synthesized iron oxide nanoparticle (IONPs) are preferred due to higher biocompatibility, non toxicity, cost effective, high yield, less energy consumption, environment friendly by products, less usage of chemicals and eco friendly nature. Green synthesis uses plant extracts (roots, leaves, stem, flowers and fruits) and microorganism (bacteria, fungus and algae) for the iron oxide nanoparticle (IONPs) development. Synthesis of plant mediated nanoparticles has obtained more notice in recent times. In this research, synthesis of iron oxide nanoparticles with desired properties has done by green synthesis method using Amaranthus Campestris plant stem extract and chemical method. This article summarizes the synthesis process and characterizations of obtained iron oxide nanoparticle (IONPs) through chemical and biological route. The comparisons between chemically and green synthesized magnetic iron oxide nanoparticle have done using various characterization methods such as FTIR, FE-SEM, XRD,VSM, UV visible Spectroscopy and antimicrobial activity.
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