Objective The aim of the present study was to assess DNA oxidative damage by detection of 8-hydroxydeoxyguanosine (8-OHdG), lipid peroxidation, and antioxidant status in thalassemic patients.Materials and methods Blood samples were collected from 94 participants (64 b-thalassemic patients and 30 healthy controls) in the range of ages 4-15 years.Results It was clear that markers of free oxygen radical injury in blood (i.e. 8-OHdG) were significantly elevated in b-thalassemia major (P < 0.001) and b-thalassemia intermediate (P < 0.001) compared with normal controls, and lipid peroxide was also significantly higher in both types of thalassemia compared with controls (P < 0.001). In contrast, mean catalase levels and glutathione-Stransferase were decreased (P < 0.001). There were highly significant changes in the values of 8-OHdG between the thalassemia major and thalassemia intermedia groups (P = 0.000), and it was significantly correlated with serum ferritin in thalassemic children (r = 0.5, P = 0.006).Conclusion b-Thalassemic children are at higher risk for tissue injury and DNA oxidative damage due to a state of enhanced oxidative stress.
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