Summary 1.The need for sensitive biological measures of aquatic ecosystem integrity applicable at large spatial scales has been highlighted by the implementation of the European Water Framework Directive. Using fish communities as indicators of habitat quality in rivers, we developed a multi-metric index to test our capacity to (i) correctly model a variety of metrics based on assemblage structure and functions, and (ii) discriminate between the effects of natural vs. human-induced environmental variability at a continental scale. 2. Information was collected for 5252 sites distributed among 1843 European rivers. Data included variables on fish assemblage structure, local environmental variables, sampling strategy and a river basin classification based on native fish fauna similarities accounting for regional effects on local assemblage structure. Fifty-eight metrics reflecting different aspects of fish assemblage structure and function were selected from the available literature and tested for their potential to indicate habitat degradation. 3. To quantify possible deviation from a 'reference condition' for any given site, we first established and validated statistical models describing metric responses to natural environmental variability in the absence of any significant human disturbance. We considered that the residual distributions of these models described the response range of each metric, whatever the natural environmental variability. After testing the sensitivity of these residuals to a gradient of human disturbance, we finally selected 10 metrics that were combined to obtain a European fish assemblage index. We demonstrated that (i) when considering only minimally disturbed sites the index remains invariant, regardless of environmental variability, and (ii) the index shows a significant negative linear response to a gradient of human disturbance. 4. Synthesis and applications . In this reference condition modelling approach, by including a more complete description of environmental variability at both local and regional scales it was possible to develop a novel fish biotic index transferable between catchments at the European scale. The use of functional metrics based on biological attributes of species instead of metrics based on species themselves reduced the index sensitivity to the variability of fish fauna across different biogeographical areas.

Fish assemblage indicators developed throughout the world were reviewed and key differences in methodologies depending on ecoregions, basins and contrasting fish fauna summarised. Common elements of existing Indices of Integrity were identified to support the development of a European-wide fish index. These include, using reference condition, accounting for natural fish assemblage variability, evaluating metric precision and selecting the most sensitive and complementary metrics. For future developments, it was recommended to pay more attention to temporal variability in fish assemblages, age structure of key (sentinel) species and fish migration. Testing hypotheses at different steps of the process seems to be the appropriate way to design fish indices.

The functional ecological guild approach is the cornerstone for the development of Indices of Biotic Integrity and multi-metric indices to assess the ecological status of aquatic systems. These indices combine metrics (unit-specific measures of a functional component of the fish community known to respond to degradation) into a single measure of ecological assessment. The guild approach provides an operational unit linking individual species characteristics with the community as a whole. Species are grouped into guilds based on some degree of overlap in their ecological niches, regardless of taxonomic relationships. Despite European fish species having been classified into ecological guilds, classification has not been standardised Europe-wide or within the context of classifying species into guilds from which metrics can be developed for ecological assessment purposes. This paper examines the approach used by the EU project FAME to classify European fish species into consistent ecological guilds and to identify suitable metrics as basic tools for the development of a standardised ecological assessment method for European rivers to meet the requirements of the Water Framework Directive.

This telemetry study aimed to document the mobility of Salmo trutta in the River Ourthe sub-basin (tributary of the River Meuse) during summer and autumn, and to analyse the environmental factors which trigger spawning migration or limit their extension. Nine trout (233-2217 g and 26.6-55.2 cm FL) were radio-tagged with intraperitoneal radio transmitters and positioned daily, from 14 August 1996 to 15 January 1997. Until 1 October, fish showed restricted movements: daily journeys never exceeded 300 m and corresponded to displacements by high floods or to routine home range movements. From 7 October to 15 November, seven of the nine trout travelled upstream over distances from 5.6 to 22.95 km, into tributaries and sub-tributaries. Migration speed was fast during the early days, when trout could travel over more than 5 km per night, then progressively decreased as they were approaching putative spawning redds under lower temperature. Both in the River Ourthe and in the Aisne stream, all migrations started within less than three weeks (early October) and were found to be triggered by the combination of three environmental factors: high variations of water temperature and water level between consecutive days, within a thermal range of 10-12 • C. From the trout point's of view, these may be signs that the environment becomes unpredictable, as its variability increases within a thermal range which no longer enables them to achieve high growth rates. These results are discussed within the context of foraging strategies, life history strategies and management of trout population.

The objective was to develop spatially based (type-specific) methods to assess the ecological status of European rivers according to the EU Water Framework Directive. Some 15 000 samples from about 8000 sites were pre-classified within a five-tiered classification system based on hydromorphological and physico-chemical pressures. The pre-classification was used to identify reference conditions and to calibrate the assessment methods. Clustering reference sites based on relative species composition resulted in 60 fish assemblage types within 11 of the ecoregions under study. Discriminant function analyses (DFAs) were employed to identify environmental parameters characterising fish assemblage types; altitude, river slope, wetted width, mean air temperature and distance from source were the principal predictors. These environmental parameters were used to assign impacted sites with altered fish assemblage composition to the reference fish assemblage type. Metrics (fish assemblage descriptors) responding to human pressures were selected based on correlation and DFAs. Assessment methods were developed for 43 fish assemblage types. Metrics based on individual sentinel species were more often used in type-specific methods than metrics related to reproduction, habitat and feeding. Metrics based on long-distance migrants and potamodromous species were more sensitive to human pressures than overall composition metrics, e.g. total number of species. Only some of the tested metrics showed pressure-specific responses, i.e. reacted to one type of pressure but not to others. Insectivorous, intolerant and lithophilic species exclusively responded (decreased) to chemical and hydromorphological pressures in 14-19%. Omnivorous species was the only metric type that showed a consistent reaction (increase) to continuum disruptions in 25% of the cases. Accuracy of methods based on cross-validation with pre-classification varied between 47% and 98% (mean 81%) when contrasting calibration data set (class 1 and 2) with degraded sites (class 3, 4 and 5).