In this work we have replaced a commonly used Au film with an Au/Ag bimetallic film working as the sensing chip in the commercial surface plasmon resonance (SPR) instrument, Biacore 3000. The bimetallic chip gives out sharper SPR dip than that by the chip made of an Au film. The applicability and stability of the bimetallic chip on the Biacore 3000 are tested by real-time monitoring of biological coupling processes between sulfamethoxazole (SMX) and anti-SMX, which is useful in rapid detection of contaminations in food products. The bimetallic chip performs stably in 6 times' usage and regeneration. Our experiment is helpful to push the bimetallic film into real applications in commercial SPR instruments.
In this paper, leakage radiation microscopy (LRM) was used to characterize the surface
plasmon coupled emission (SPCE) and waveguide mode coupled emission (WMCE).
Rhodamine (RhB) molecules doping PMMA films with different thicknesses were
spin-coated onto silver films deposited on a glass substrate, which were used to
generate both the SPCE and WMCE. A numerical simulation was carried out and
was consistent with the experimental results, which verifies the feasibility of this
method. When compared with a prism-based set-up, LRM has the advantages
of getting the emitting angle without scanning and higher spatial resolution.
In this letter, we report the experimental results on plasmonic waves excited on a silver film coated with dye-doped poly(methyl methacrylate) nanogratings, which are characterized by leakage radiation microscopy. Various patterns of the surface plasmon-coupled emission are demonstrated with the structured nanogratings. Plasmonic waves of anisotropic wave-numbers and Bragg mirror effect are both observed in this configuration. Mechanism of the experimental results is discussed from the viewpoint of coupling between adjacent stripe loaded surface plasmons. Our work has potential application in the development of plasmon lasers.
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