Soybean and canola proteins were treated with heat, formaldehyde (HCHO), sodium hydroxide (NaOH), whole fresh blood (BL) or fish hydrolysate (FH). Effect of these treatments on in situ protein degradability was measured by the nylon bag technique using fisulated steers. All treatments with the exception of heat were effective in protecting protein of soybeans and soybean meal (SBM). Canola seed protein was protected with heat at 110 °C for 2 h and with chemical treatments; all treatments were effective in protecting canola meal (CM) protein. Essential amino acid profiles of these residues from HCHO- or NaOH-treated SBM were not different from untreated SBM residue but increases in some essential amino acids were found in BL-treated SBM residue. Rat bioassays of residues from nylon bag studies revealed that protein quality of SBM, as indicated by net protein ratio, was significantly reduced (P < 0.05) by addition of HCHO, NaOH, BL or FH. Protein digestibility of SBM and CM was decreased by HCHO treatment but not by other treatments. NaOH treatment of CM had no effect on protein quality but HCHO, BL or FH treatments significantly improved (P < 0.05) protein quality of CM residue compared with untreated CM residue. These studies demonstrate that soybean and canola proteins can be effectively protected from degradation in the rumen by NaOH, BL or FH treatment without adverse effect on protein digestibility. Key words: Formaldehyde, sodium hydroxide, blood, fish hydrolysate, soybean, canola
In trial 1, the effects of dietary energy (102, 131 or 162% of requirement) in the dry period and of sodium bicarbonate (0 or .75% of diet dry matter) in early lactation were assessed with 31 cows in a 3 X 2 factorial arrangement of treatments. Body condition and weight increased linearly with prepartum energy. Dry matter intake and milk yield were similar across treatments through 12 wk postpartum. Sodium bicarbonate increased milk fat content only in the 131% group, an effect apparently related to greater mobilization of fat in that group. In trial 2, energy treatments imposed in late lactation (145 to 55 d prepartum) and in the dry period (55 to 0 d) were 1) cows fed to requirement in both periods, 2) cows overfed in the first and underfed in the second period, 3) cows fed to requirement in the first and overfed in the second period, and 4) cows overfed in both periods. Cows in treatments 1 and 2 (normal) calved in a thinner state than those in 3 or 4 (fat). In the first 12 wk postpartum, intake did not differ, but cows in groups 3 and 4 produced more milk. Sodium bicarbonate imposed factorially postpartum increased milk fat content. Overconsumption of energy prepartum did not impair production when high energy total mixed rations were fed postpartum.
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