Abstraet--O-Pentafluorobenzyloxime (OPFB)-heptafluorobutyrylester (HFB)derivatives and OPFB-O-methyloxime (MO)-trimethylsilylether (TMS) derivatives of non-eedysteroid steroids were prepared from haemolymph extracts of last instar larvae of the fleshfly Sarcophaga bullata. Using a negative ion chemical ionization capillary gas chromatography-mass spectrometry (NCI/GC-MS) technique the following steroids could be identified: progesterone, testosterone, 5~t-androstane3fl,17fl-diol, 5fl-androstane-3~t,17fl-diol, androst-5-ene-3fl,17fl-diol, androstenedione, 5ct-dihydrotestosterone, ll-ketotestosterone, llfl-hydroxytestosterone, 17~t-hydroxyprogesterone, 17~t,20fl-dihydroxyprogesterone. Although the technique is very sensitive, estrogens could not be detected. These results suggest an active metabolism of progesterone and testosterone.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.