Chemical pollution and reproductive toxicity has a close association leading into a possible infertility. Testicular toxicity induced by chemicals is easy to evaluate since it has a pool of differentiating cells of different stages in the seminiferous epithelium. Most of the pesticide related preclinical studies have heavily dependent upon investigations on sperm density, abnormal sperm percent, reproductive hormone levels etc. Most of the toxins, in addition may affect the seminiferous tubular architecture, harmony as well as supporting cells such as Sertoli cells. Diazinon and its adverse sperm toxic effects are documented earlier but its effects on seminiferous tubules are less recorded. The present study was designed to evaluate the effect of oral administration of diazinon (6mg, 7.5mg and 10mg/kg body weight/day for 5 and 30days) on acetylcholine esterase activity, histological aspects of testes and seminiferous tubular morphometry (STD & SEH). Acetylcholine esterase activity was significantly decreased as a sign of toxicity, the dose tested. The STD and SEH were significantly decreased in all the 30days diazinon exposed groups. Histologically testes showed vacuoles, disruption and sloughing of basal germinal epithelium. This study confirms that diazinon disrupts cytoarchitectural components which may interfere with spermatogenesis.
Aim:To evaluate the pathogenesis in heart and liver by the early induction of biochemical and antioxidant derangements in rats exposed to endosulfan.Materials and Methods:Wistar rats were gavaged with endosulfan (0.5, 1 and 1.5 mg/kg body weight in sunflower oil) for a period of 21 days (single dose at 24 h interval). Control and sunflower oil control groups were also maintained simultaneously. Rats were sacrificed on the 22nd day posttreatment. Blood samples, heart and liver were collected and different biochemical parameters such as total protein, cholesterol, triglycerides, amino acids and antioxidant and lipid peroxidation level were measured. Statistical analysis was carried out by one way ANOVA, followed by Bonferroni’ post-hoc test.Results:Endosulfan induced a significant increase in the serum levels of total protein, amino acids, triglyceride, total cholesterol, free fatty acid and phospholipid levels in a dose-dependent manner. In the heart and liver, lipid peroxidation was increased significantly in a dose-dependent manner and the antioxidant levels such as superoxide dismutase (SOD), glutathione S-transferase, glutathione peroxidase, and catalase were significantly decreased in a dose-dependent pattern.Conclusion:Exposure to endosulfan results in a significant derangement in the biochemical parameters with a decrease in antioxidant levels in the heart and liver. This is an early indication of pathogenesis in the vital organs of rats.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.