The present investigation had its origin in the suggestion that the blood of the emergent vein of the organ or organs in which platelet production is taking place should show a higher platelet count than the venous blood of other organs. In order to carry out this idea it was essential to find a satisfactory method of counting both platelets and erythrocytes in samples of blood removed from the veins by means of a syringe. Such a method was devised. It is described in detail below. In applying this method to various arteries and veins it was found that the platelet count, relative to the number of erythrocytes, was, in general, higher in the arteries t h a n in the veins, indicating an increase in the n u m b e r of platelets during the passage of the blood through the lungs. This point, therefore, was made the subject of a special study b y experimental and histological methods, the results of which are given in this paper. MethodsRemoval of Samples of Blood.--For this purpose 0.1 cc. of blood was taken from an artery or vein by means of a 0.25 cc. tuberculin syringe (B-D). The syringes used were calibrated with mercury, but the graduation as given by the makers was found to be correct. In these syringes the 0.25 cc. is divided into 25 equal parts, each representing 0.01 cc. In taking a sample of blood the syringe with needle attached was filled with the diluting mixture to mark 15. The needle (No. 24) was then inserted into the vessel and blood was drawn to mark 25, giving, therefore, 0.1 cc. of blood. The contents of the syringe were emptied into 39 cc. of the diluting mixture and the syringe was filled and emptied three times with the diluting mixture, so that the final dilution of the blood was 0.1 cc. in 40 cc. of the mixture, or 1 to 400. This mixture was stirred thoroughly for 5 minutes with an electric stirrer, the wide paddies of which were coated with bakelite varnish, and samples were then taken for the counting chamber by means of an appropriate 177
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