Expression of optogenetic tools in surviving inner retinal neurons to impart retinal light sensitivity has been a new strategy for restoring vision after photoreceptor degeneration. One potential approach for restoring retinal light sensitivity after photoreceptor degeneration is to express optogenetic tools in retinal ganglion cells (RGCs). For this approach, restoration of ON and OFF center-surround receptive fields in RGCs, a key feature of visual information processing, may be important. A possible solution is to differentially express depolarizing and hyperpolarizing optogenetic tools, such as channelrhodopsin-2 and halorhodopsin, to the center and peripheral regions of the RGC dendritic field by using protein targeting motifs. Recombinant adeno-associated virus (rAAV) vectors have proven to be a powerful vehicle for in vitro and in vivo gene delivery, including in the retina. Therefore, the search for protein targeting motifs that can achieve rAAV-mediated subcellular targeted expression would be particularly valuable for developing therapeutic applications. In this study, we identified two protein motifs that are suitable for rAAV-mediated subcellular targeting for generating center-surround receptive fields while reducing the axonal expression in RGCs. Resulting morphological dendritic field and physiological response field by center-targeting were significantly smaller than those produced by surround-targeting. rAAV motif-mediated protein targeting could also be a valuable tool for studying physiological function and clinical applications in other areas of the central nervous system.

Quantum fluctuations of electromagnetic radiation pressure are discussed. We use an approach based on the quantum stress tensor to calculate the fluctuations in velocity and position of a mirror subjected to electromagnetic radiation. Our approach reveals that radiation pressure fluctuations are due to a cross term between vacuum and state dependent terms in a stress tensor operator product. Thus observation of these fluctuations would entail experimental confirmation of this cross term. We first analyze the pressure fluctuations on a single, perfectly reflecting mirror, and then study the case of an interferometer. This involves a study of the effects of multiple bounces in one arm, as well as the correlations of the pressure fluctuations between arms of the interferometer. In all cases, our results are consistent with those previously obtained by Caves using different mehods.

We discuss the effect of quantum stress tensor fluctuations in deSitter spacetime upon the expansion of a congruence of timelike geodesics. We treat a model in which the expansion fluctuations begin on a given hypersurface in deSitter spacetime, and find that this effect tends to grow, in contrast to the situation in flat spacetime. This growth potentially leads to observable consequences in inflationary cosmology in the form of density perturbations which depend upon the duration of the inflationary period. In the context of our model, the effect may be used to place upper bounds on this duration.

The quantum Brownian motion of a charged particle in the electromagnetic vacuum fluctuations is investigated near a perfectly reflecting flat boundary, taking into account the smooth switching process in the measurement.Constructing a smooth switching function by gluing together a plateau and the Lorentzian switching tails, it is shown that the switching tails have a great influence on the measurement of the Brownian motion in the quantum vacuum. Indeed, it turns out that the result with a smooth switching function and the one with a sudden switching function are qualitatively quite different. It is also shown that anti-correlations between the switching tails and the main measuring part plays an essential role in this switching effect.The switching function can also be interpreted as a prototype of an non-equilibrium process in a realistic measurement, so that the switching effect found here is expected to be significant in actual applications in vacuum physics.