Analyses of inflammation inducing Th cells revealed that a portion of these cells express both IFN‐γ and IL‐17, suggesting that Th1 and/or Th17 may switch their phenotype. Here, we examined phenotype retention in a system in which polarized Th1 or Th17 cells specific against hen egg lysozyme (HEL) induce ocular inflammation in recipient mice expressing HEL in their eyes. Whereas no switching to Th17 was detected in Th1 recipient eyes, substantial proportions of transferred Th17 expressed IFN‐γ, or both IFN‐γ and IL‐17 in Th17 recipient eyes. The phenotype switch of Th17 cells increased with time and was attributed to exposure to IL‐12 that is expressed in the inflamed eyes. Incubation in vitro with Th1 polarizing cocktail (IL‐2+IL‐12) converted most Th17 into IFN‐γ or IFN‐γ/IL‐17 expressing cells, but no reciprocal conversion was noted with Th1 cells. Furthermore, whereas Th1 cells did not express ROR‐γt following incubation with Th17 polarizing cocktail, Th17 expressed T‐bet following incubation with Th1 cocktail. Thus, polarized Th1 cells fully retain their phenotype, while Th17 switch and express IFN‐γ or both IFN‐γ and IL‐17, as well as T‐bet, following exposure to IL‐12. This study is supported by NEI/NIH intramural program.

The technical breakthrough in synthesizing graphene by chemical vapor deposition methods (CVD) has opened up enormous opportunities for large-scale device applications. In order to improve the electrical properties of CVD graphene grown on copper (Cu-CVD graphene), recent efforts have focussed on increasing the grain size of such polycrystalline graphene films to 100 micrometers and larger. While an increase in grain size and hence, a decrease of grain boundary density is expected to greatly enhance the device performance, here we show that the charge mobility and sheet resistance of Cu-CVD graphene is already limited within a single grain. We find that the current high-temperature growth and wet transfer methods of CVD graphene result in quasi-periodic nanoripple arrays (NRAs).Electron-flexural phonon scattering in such partially suspended graphene devices introduces anisotropic charge transport and sets limits to both the highest possible charge mobility and lowest possible sheet resistance values. Our findings provide guidance for further improving the CVD graphene growth and transfer process.KEYWORDS CVD graphene, quasi-periodic nanoripple arrays, anisotropic, Charge transport, flexural phonon scattering, transparent electrodes, sheet resistance Graphene 1 is a promising material for many novel device applications such as ultrafast nanoelectronics, optoelectronics and flexible transparent electronics. [2][3][4][5] Cu-based CVD methods have now made wafer-scale graphene synthesis and transfer feasible both for single layer graphene 6,7 (SLG) and bilayer graphene (BLG). 8 This not only brings the commercial applications of graphene within reach, but also provides great advantages in introducing new substrates to enhance and engineer its electronic properties by tuning the substrate-induced screening 9-12 and substrate-induced strain. 13,14 Unlike CVD graphene growth on Ni, 15,16 Cu-CVD graphene growth has a rather weak interaction with the underlying Cu substrate, allowing CVD graphene to grow continuously crossing atomically flat terraces, step edges, and vertices without introducing significant defects. 17 Thus, by controlling pregrowth annealing 7 and fine tuning growth parameters, 18,19 it is now possible to synthesize CVD 3 graphene with sub-millimetre grain size. However, pre-growth annealing and CVD growth typically require high temperatures very close to the melting point of Cu at 1083 ºC. This leads to Cu surface reconstruction and local surface melting 17,20 during graphene growth, making high density Cu singlecrystal terraces and step edges ubiquitous surface features. Taking into account the negative thermal expansion coefficient of graphene, this leads to new surface corrugations in CVD graphene during the cool down process. 21 Previously grain boundaries have been identified as one of the limiting factors to degrade graphene quality. 22 While the heptagon and pentagon network 22,23 at grain boundaries does disrupt the sp 2 delocalization of π electrons in graphene, it remains to be se...

MicroRNAs (miRNAs) play important regulatory roles in animals and plants by targeting mRNAs for cleavage or translational repression. They have diverse expression patterns and might regulate various developmental and physiological processes. Profiling miRNA expression is very helpful for studying biological functions of miRNAs. We report a novel miRNA profiling microarray, in which miRNAs were directly labeled at the 3′ terminus with biotin and hybridized with complementary oligo-DNA probes immobilized on glass slides, and subsequently detected by measuring fluorescence of quantum dots labeled with streptavidin bound to miRNAs through streptavidin–biotin interaction. The detection limit of this microarray for miRNA was ∼0.4 fmol, and the detection dynamic range spanned about 2 orders of magnitude. We made a model microarray to profile 11 miRNAs from leaf and root of rice (Oryza sativa L. ssp. indica) seedlings. The analysis results of the miRNAs had a good reproducibility and were consistent with the northern blot result. To avoid using high-cost detection equipment, colorimetric detection, a method based on nanogold probe coupled with silver enhancement, was also successfully introduced into miRNA profiling microarray detection.

Abstract. A high O 3 episode with the large increases in surface ozone by 21-42 ppbv and the nocturnal surface O 3 levels exceeding 70 ppbv was observed in the region between Xiamen and Quanzhou over the southeastern coast of China during 12-14 June 2014, before the Typhoon Hagibis landing. Variations in the surface O 3 , NO 2 , CO and meteorology during the Typhoon Hagibis event clearly suggest a substantial impact of the peripheral downdrafts in the large-scale typhoon circulation on such an O 3 episode excluding the contributions of photochemical production and the horizontal transport. The influence of vertical O 3 transport from the upper troposphere and lower stratosphere (UTLS) region on high surface O 3 levels is further confirmed by a negative correlation between surface O 3 and CO concentrations as well as dry surface air observed during the O 3 episode. This study provides observational evidence of typhoon-driven intrusion of O 3 from the UTLS region to surface air, revealing a significant effect of such a process of stratosphere-troposphere exchange (STE) of O 3 on tropospheric O 3 and ambient air quality.