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The toxicity of nanomaterials to microorganisms is related to their dose and environmental factors. The aim of this study was to investigate the shifts in the microbial community structure and metabolic profiles and to evaluate the environmental factors in a laboratory scale intertidal wetland system exposed to zinc oxide nanoparticles (ZnO NPs). Microbial assemblages were determined using 16S rRNA high-throughput sequencing. Community-level physiological profiles were determined using Biolog-ECO technology. Results showed Proteobacteria was the predominant (42.6%–55.8%) phylum across all the sediments, followed by Bacteroidetes (18.9%–29.0%). The genera Azoarcus, Maribacter, and Thauera were most frequently detected. At the studied concentrations (40 mg·L−1, 80 mg·L−1, 120 mg·L−1), ZnO NPs had obvious impacts on the activity of Proteobacteria. Adverse effects were particularly evident in sulfur and nitrogen cycling bacteria such as Sulfitobacter, unidentified_Nitrospiraceae, Thauera, and Azoarcus. The alpha diversity index of microbial community did not reflect stronger biological toxicity in the groups with high NP concentrations (80 mg·L−1, 120 mg·L−1) than the group with low NP concentration (40 mg·L−1). The average well color development (AWCD) values of periodically submersed groups were higher than those of long-term submersed groups. The group with NP concentration (40 mg·L−1) had the lowest AWCD value; those of the groups with high NP concentrations (80 mg·L−1, 120 mg·L−1) were slightly lower than that of the control group. The beta diversity showed that tidal activity shaped the similar microbial community among the periodically submerged groups, as well as the long-term submerged groups. The groups with high DO concentrations had higher diversity of the microbial community, better metabolic ability, and stronger resistance to ZnO NPs than the groups with a low DO concentration.

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As an environment-friendly wastewater treatment process, the anaerobic fermentation process has been widely used for the pretreatment of high-strength wastewater. However, it is rarely applied to treat low-strength wastewater due to low methane recovery. In this study, anaerobic fermentation treating low-strength mariculture wastewater was studied in an anaerobic sequencing batch reactor (ASBR) with a COD removal rate of 75%. Anaerobic fermentation was successfully controlled at the acidification stage by increasing COD loading. As the greenhouse gas emission decreased, the residual organics were enough for biological nutrients’ removal. Fluorescence in situ hybridization results showed that the dominant bacteria in the ASBR were acidogenic bacteria and methanogens, accounting for 39.7% and 46.5% of the total bacteria, respectively. Through the calculation processing of the experimental data, the order of the anaerobic fermentation reaction was a second-order reaction. The kinetic parameters of low-strength organic wastewater treatment were determined by using the Grau second-order substrate removal model, Stover–Kincannon model, Monod model and Haldane model. The maximum rate removal constant Umax, sludge yield coefficient Y and inhibition constant Ki were 1.157 g/(L·d), 0.153 mgVSS/mgCOD and 670 mg/L, respectively. It provided data support for the practical application of the anaerobic fermentation treating low-strength wastewater.