Plasmacytoma variability translocation 1 (PVT1), an oncogene, has been reported to be highly expressed in many tumors, including human glioma, gastric cancer, and non-small cell lung cancer. Functionally, it could also regulate the development of tumor cells. However, its specific roles and pathogenesis in human gliomas are still not clear. This study investigated the function and mechanism of PVT1 knockdown in the proliferation and malignant transformation of human gliomas. We first examined the expression levels of PVT1 and miR-424 in human glioma tissues and cell lines. We also used gene manipulation techniques to explore the effects of PVT1 knockdown on cell viability, migration, invasion, and miR-424. We found that PVT1 knockdown effectively inhibited cell viability, migration, and invasion of human glioma cells and increased miR-424 expression. Based on the negative correlation between PVT1 and miR-424, we then confirmed the direct interaction between PVT1 and miR-424 using RNA immunoprecipitation (RIP) and luciferase reporter assays. Further, we established a xenograft nude mouse model to determine the role and mechanism of PVT1 on tumor growth in vivo. In addition, PVT1 knockdown was shown to promote miR-424 in vivo. In summary, the present study demonstrated that PVT1 knockdown could negatively regulate miR-424 to inhibit human glioma cell activity, migration, and invasiveness. PVT1 knockdown could negatively regulate miR-424 to inhibit cellular activity, migration, and invasiveness in human gliomas, which explained the oncogenic mechanism of PVT1 in human gliomas. It also suggested that PVT1 might be a novel therapeutic target for human gliomas.

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Exogenous carbon (C) not only regulates plant growth but also provides energy for microbes and improves the soil environment. We hypothesised that exogenous C could improve plant growth by affecting the soil environment. Therefore, pot experiments were conducted and peanut cvs. Huayu 22(H) and NN-1(B) were used under three different treatments (the control, single nitrogen (N), and N combined with glucose (CN)). The results showed that the abundance and diversity of N-fixing bacteria are obviously influenced by the C and N, and exogenous C can promote the restoration of microbial diversity. The relative abundances of Burkholderiales were increased under HCN and BCN to 9.8% and 9.5%, respectively, compared to the control (3.9%, 2.5%). The abundance of N fixation bacteria increased mainly due to the soil nutrient change. In comparison with the single N treatment, the addition of the C significantly decreased the soil NH4+-N and NO3–-N contents by 31.0% and 13.3%, respectively. And the activities of soil urease and nitrogenase were significantly increased. Compared to the control, single N significantly limited the root development, while the addition of C played a promoting role in root growth. Plant N accumulation increased compared with the control, but there was no significant difference between N treatment and CN treatment. These results indicated that exogenous C promoted soil microorganism activity and strengthened plant growth by changing the soil environment.

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