Nasopharyngeal carcinoma (NPC) is the most common cancer among head and neck cancers in Vietnam. We aimed to identify the rate of a 30 bp deletion mutation of the LMP1-EBV gene in nasopharyngeal biopsy tissue samples, the HLA genotypes of NPC patients, and the relationship between these two targets. Patients with NPC at Can Tho Oncology Hospital from September 2014 to December 2018 were selected. A length of 30 bp of the del-LMP1-EBV gene was analyzed using a PCR technique, and the HLA genotypes in patients’ blood samples were analyzed with PCR-SSO technology. HLA-B*15 gene carriers had the highest risk of 30 bp LMP1-EBV gene deletion mutation, which was found in 51 out of 70 patients (72.9%). Carriers of the HLA-B*15 allele had a 4.6-fold increased risk of a 30 bp del-LMP1-EBV gene compared with non-carriers of this allele. The initial identification of NPC was related to the 30 bp del-LMP1-EBV gene and high frequencies of the -A*02, -B*15, -DRB1*12, -DQB1*03, and -DQA1*01 HLA alleles. Our study results suggest an association of the 30 bp del-LMP1-EBV gene and the HLA-B*15 allele with NPC susceptibility.
Background: Drug resistance has become a major concern for antiviral therapy in patients with chronic hepatitis B (CHB), leading to an increased risk of treatment failure. We aimed to identify the prevalence of hepatitis B virus (HBV) DNA polymerase mutations and to determine the frequency of HBV genotypes and subgenotypes in treatment-naive patients with CHB in Mekong Delta, Vietnam.Methods: Of the 550 drug-naive CHB patients from Mekong Delta, their serum samples were analyzed for HBV DNA mutations, genotypes, and subgenotypes using polymerase chain reaction sequencing.Results: Of the 550 patients who were infected with either HBV genotype B (72.9%) or genotype C (27.1%), the frequencies of subgenotypes were 19% B1, 3.6% B2, 10.3% B3, 39.8% B4, 0.4% C1, and 26.9% C2. The only mutation that was detected in 54 patients (10.7%) was rtV207M. The rtV207M mutation was found among 13.9% (51/368) of genotype B and among 2.2% (3/137) of genotype C (P < 0.0001).Discussion: Genotype B and subgenotype B4 are predominant in Mekong Delta. Only 1 mutation rtV207M is discovered in our study. These findings suggest that it is unnecessary to test for HBV resistance mutation before delivering antiviral therapy.
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