European mustelids include the European polecat, Mustela putorius, and the steppe polecat, M. eversmanii. Both occur sympatrically in the Pannonian Basin, where M. eversmanii hungarica represents the westernmost part of the latter species and they allegedly hybridize. We investigated the morphological relationships in sympatric and allopatric populations of these mustelids with representative sampling, taxonomic and geographic coverage. We evaluated inter- and intraspecific patterns of morphological differentiation of 20 cranial measurements and four external traits by distance-based morphometric approaches and multivariate analyses. Our results revealed a considerable heterogeneity in cranial morphology. The two species appeared to be clearly differentiated although sympatric populations were closer to each other and had a slight overlap in the morphometric space. Within M. eversmanii, the subspecies and the nominal taxon only partially overlapped, and M. eversmanii eversmanii was more distant from M. putorius than subspecies hungarica. Although morphometric analyses revealed several intermediate individuals in size in sympatric M. eversmanii and M. putorius populations, only a small fraction of such specimens showed conflict in discrete morphological characters with the diagnostic discriminant function. We interpret these results as an indication of ongoing hybridisation between sympatric populations, but the low number of hybrids identified suggests limited genetic exchange between the species.

Temporal activity differences facilitate species’ coexistence by reducing interspecific competition. Such patterns can be studied via diel activity analysis, but obtaining data in cryptic mammals is difficult. We investigated the annual and diel activity pattern of such a small mammal, the endangered Hungarian birch mouse (Sicista trizona trizona), in its only known habitat. We employ trail cameras for the first time to reveal the diel and annual activity of a sminthid species. Data acquisition included the spring and summer seasons between 2019 and 2022 and was extended to detect the activity overlaps with other common coexisting rodents and shrews. The diel activity results rely on 581 detections of S. trizona over 5670 trap-nights of camera trap deployment characterising also activity pattern of the small mammal community in this Central European grassland ecosystem. S. trizona was not recorded during the day but was active at dawn and night, and in comparison with other coexisting species, its activity level was high. The presumed cold sensitivity was not confirmed as we detected activity at −6 °C. Diel activity peaked in early May in the mating season. Although the diel activity pattern of co-occurring small mammal species was also nocturnal, activity overlaps were relatively high suggesting that temporal niche partitioning is limited within the habitat. Our work provides the first insight into temporal overlaps within a small mammal community in a natural European grassland, moreover, also the first documented research on the activity pattern of a sminthid in its natural habitat.