Cyclooxygenase (COX) is an enzyme involved in tumorigenesis and is associated with tumor cell resistance against platinum-based antitumor drugs. Cisplatin analogues were conjugated with COX inhibitors (indomethacin, ibuprofen) to study the synergistic effects that were previously observed in combination treatments. The conjugates ensure concerted transport of both drugs into cells, and subsequent intracellular cleavage enables a dual-action mode. Whereas the platinum(II) complexes showed cytotoxicities similar to those of cisplatin, the platinum(IV) conjugates revealed highly increased cytotoxic activities and were able to completely overcome cisplatin-related resistance. Although some of the complexes are potent COX inhibitors, the conjugates appear to execute their cytotoxic action via COX-independent mechanisms. Instead, the increased lipophilicity and kinetic inertness of the conjugates seem to facilitate cellular accumulation of the platinum drugs and thus improve the efficacy of the antitumor agents. These conjugates are important tools for the elucidation of the direct influence of COX inhibitors on platinum-based anticancer drugs in tumor cells.
Prostaglandins (PGs) are powerful lipid mediators in
many physiological
and pathophysiological responses. They are produced by oxidation of
arachidonic acid (AA) by cyclooxygenases (COX-1 and COX-2) followed
by metabolism of endoperoxide intermediates by terminal PG synthases.
PG biosynthesis is inhibited by nonsteroidal anti-inflammatory drugs
(NSAIDs). Specific inhibition of COX-2 has been extensively investigated,
but relatively few COX-1-selective inhibitors have been described.
Recent reports of a possible contribution of COX-1 in analgesia, neuroinflammation,
or carcinogenesis suggest that COX-1 is a potential therapeutic target.
We designed, synthesized, and evaluated a series of (E)-2′-des-methyl-sulindac sulfide (E-DMSS) analogues for inhibition of COX-1. Several potent
and selective inhibitors were discovered, and the most promising compounds
were active against COX-1 in intact ovarian carcinoma cells (OVCAR-3).
The compounds inhibited tumor cell proliferation but only at concentrations
>100-fold higher than the concentrations that inhibit COX-1 activity. E-DMSS analogues may be useful probes of COX-1 biology in
vivo and promising leads for COX-1-targeted therapeutic agents.
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