Graphical AbstractFig. 1. Fresh Vitis leaves, roots and grapes from the Organic Garden at St. Thomas University.Abstract. Vitis rotundifolia (muscadine grape) is a grapevine species found in humid subtropical climates. Previous researches have reported the bioactivity of the muscadine grapes and our purpose is to look for valuable compounds from plants with medicinal properties or health benefits. The plant used in this research was collected in the organic garden at St. Thomas University. Muscadine plant extracts were analyzed by TLC (Thin Layer Chromatography) using polar and non-polar solvents, and the spots were visualized with Iodine and UV light. The antioxidant activity assays were performed with the extracts of oven and freeze-dried leaves, grapes, and roots. The free radical scavenging activity (FRS) was evaluated with the DPPH reagent, and is reported as ascorbic acid equivalent antioxidant capacity, measured spectrophotometrically at 517 and 520 nm. The estimation of the ferric reducing power (FRP) with potassium ferricyanide was obtained, and the absorbances of the colored complex in the extracts were taken at 700 nm. Total Phenolic Content (TPC) assay of the samples using Folin-Ciocalteu reagent for all parts of the plant showed up the grapes exhibiting the highest antioxidant content compare with the leaves and roots.
Graphical AbstractFig. 1. Braxilian Pepper Tree: leaves, bark and berries from the Organic Garden at St. Thomas University.Abstract. In the present study, Schinus Terebinthifolius (Brazilian Pepper Tree) extracts were evaluated for antioxidant activity using free radical scavenging activity, and ferric reducing power. The plant was collected in the organic garden at St. Thomas University and the extracts were prepared by maceration of three parts of the plants, the leaves, the berries, and the bark. The extracts were made using varying proportions of ethanol and hexane solvents. All the samples were analyzed using thin layer chromatography (TLC). Multiple extract samples were submitted to DPPH (2,2-diphenyl-1-picrylhydrazyl) assay to determine the free radical scavenging (FRS) capacity, and absorbance was read at 517 and 520 nm in a plate reader. A control sample was prepared containing the same volume of solvent and DPPH without any extract and reference ascorbic acid. Percent scavenging activity of the DPPH free radical is expressed as an ascorbic acid (AA) equivalent antioxidant capacity (mg AA/100g). A Ferric reducing anti-oxidant power assay (FRAP) was performed and absorbance was measured at 700 nm to quantify the total antioxidant activity. FRAP and DPPH assays indicated that the bark has significantly higher free radical scavenging ability than any other part of the plant. IntroductionSchinus Terebinthifolius has been widely used in South America in herbal remedies and was reported to have anti-bacterial and antioxidant properties. High levels of antioxidant capacity in plants are believed to decrease oxidative stress and free radicals in the body..Background research of the Brazilian Pepper Tree indicated that certain parts of the plant exhibited higher antioxidant and anti-bacterial levels than others. In order to compare the antioxidant activity levels, the plant was categorized into leaves, bark, and berries for testing. Also be used to evaluate total phenolic content and concentrations of antioxidants in different parts of the vine.
;. Graphical Abstract AbstractPlants are a large source of antioxidant compounds. This project presents the determination of the antioxidant capacity of the Oyster Plant (Tradescantia Spathacea). The samples consisted of ethanol/hexane extracts of the stems, roots, and leaves. The antioxidant activity was measured by three different assays: ferric reducing anti-oxidant power assay (FRAP), DPPH free radical scavenging (FRS) method in vitro antioxidant activity and is expressed as ascorbic acid (AA) equivalent antioxidant capacity (mg AA/100g). The total phenolic content (TPC) was determined with the Folin-Ciocalteau reagent and expressed as mg/g gallic acid equivalents (GAE). This study showed that Tradescantia Spathacea extracts contain a number of health promoting bioactive compounds, such as phenolic compounds, and are potential sources of natural antioxidants. IntroductionMedicinal plants and plant derived products have been part of the health-care system since ancient human civilization. Traditional medicine is widely used, and plants are a large source of antioxidant compounds such as phenols, carotenoids, and flavonoids with potent antioxidant properties that have received much attention recently. The Oyster Plant (Tradescantia Spathacea) is a fleshy or succulent perennial garden herb ornamental plant and is found in many tropical countries. Medicinally, the plant is used for colds, sore throat, whooping cough, nasal bleeding, and is also used as an antiinflammatory. The plant was grown in the organic garden at St. Thomas University and the ethanol/hexane extracts via maceration of the roots and leaves were analyzed to measure the antioxidant activity by three different assays: Ferric reducing power (FRAP), DPPH free radical scavenging (FRS) and total phenolic content (TPC).
The Oyster plant (Tradescantia spathacea) is a fleshy or succulent perennial garden herb. It is utilized for ornamental purposes in many tropical and subtropical climates. Medicinally, the plant is used for colds, sore throat, whooping cough, nasal bleeding, and also as an anti-inflammatory. Oyster Plants were grown and harvested from the organic garden at St. Thomas University. The different parts of each plant -leaves, stems, roots and flowers -were separated, cleaned, and dried at 40°C. Specimens were then grinded and prepared as extracts using maceration and Soxhlet extraction. All the extracts were rotevapored and analyzed by thin layer chromatography (TLC) with different mixtures of polar and nonpolar solvents. The spots were developed and visualized with iodine and UV light. Root and leaf fractions contained the majority of organic compounds. The present work reports the best solvent for extraction and the most effective conditions for TLC separation. Preliminary experiments testing ethanolcontaining extracts for anticancer properties are also discussed.
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